Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/ 4.5gms Glucose per litre w/o L-Glutamine, Calcium chloride, HEPES buffer, Sodium pyruvate and Sodium bicarbonate

Dulbecco's Modified Eagle Medium is one of the most widely used modification of Eagles medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4500mgs/L of glucose. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cel ls as well as various normal and transformed cell lines.It is with L-glutamine, 4.5gms per litre glucose, calcium chloride, sodium bicarbonate, sodium pyruvate and HEPES buffer.