Iscove’s Modified Dulbecco’s Medium (IMDM) w/ L-Glutamine and 25mM HEPES buffer w/o Glucose and Sodium bicarbonate

Iscove's Modified Dulbecco's Medium is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium contains additional amino acids, sodium selenite, sodium pyruvate, vitamins and inorganic salts. Potassium nitrate is substituted by ferric nitrate. IMDM was the first medium utilizing HEPES buffer. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. AT230 is Iscove's Modified Dulbecco's Medium with Lglutamine and 25mM HEPES buffer. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.