Dulbecco’s Modified Eagle Medium (DMEM) w/ L-Glutamine w/o D-Glucose, Sodium pyruvate and Sodium bicarbonate

Dulbecco's Modified Eagle Medium is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs glucose per litre and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4.5gms/L of glucose. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. AT195 is Dulbecco's Modified Eagle Medium with L-glutamine. It does not contain Glucose, sodium bicarbonate and sodium pyruvate.