SFRE Medium 199-2 w/ Earle’s salts, L-Glutamine, Galactose and Glucose w/o Sodium bicarbonate and Insulin

SFRE Medium 199 is modification of medium 199 developed for growth and maintenance of primary baboon kidney (Bak) cells. Both the media were formulated by supplementing medium M199 with insulin, sodium pyruvate, zinc sulfate, and increasing arginine-HCl, cysteine, cystine, L-glutamine, L-glutamic acid, glycine, histidine, tyrosine, and glucose to maximally active nontoxic concentrations. SFRE 199-2 is additionally supplemented with galactose to avoid excessive accumulation of lactic acid and to maintain pH in the physiological range for prolonged maintenance of the cells.