HiGlutaXL™ RPMI-1640 w/ L-Alanyl-L-Glutamine, HEPES buffer, 60mg per litre Penicillin, 100mg per litre Streptomycin, 15% FBS and Sodium bicarbonate (·8ml medium in 15ml round bottom centrifuge tube)

HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. It offers several advantages over the conventional glutamine containing media. Dipeptide form prevents toxic build-up of ammonia. L-alanyl-Lglutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell which obviates the need to supplement L-glutamine frequently. This media also contains antibiotics and serum. AL751G is RPMI 1640 complete medium with L-alanyl-L-glutamine, HEPES buffer, antibiotics, sodium bicarbonate and serum.