HiKaryoXL™ RPMI Medium w/ L-Glutamine, FBS, PHA-P, Penicillin, Streptomycin and Sodium bicarbonate

Cytogenetic studies include metaphase and pro-metaphase studies carried out on lymphocytes to detect chromosomal aberrations associated with structural and numerical abnormalities. Lymphocytes come from normal peripheral blood and are mitotically inactive, hence have to be stimulated with a mitogen such as Phytohemagglutinin M(PHA-M) or Phytohemagglutinin P(PHA-P). AL249A is composed of a basal medium RPMI 1640 and supplemented with L-glutamine, FBS, PHA-P, penicillin, streptomycin and sodium bicarbonate. It is a complete medium and does not require supplementation with any additional components.