Minimum Essential Medium Eagle (MEM) (For Suspension Culture) w/ Spinner salts and Sodium bicarbonate w/o L-Glutamine

Minimum Essential Medium(MEM) is a modification of Basal Medium Eagle(BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL019 is Minimum Essential Medium with Spinner’s balanced salts for suspension culture. It does not contain L-glutamine.