Acrylamide/ Bisacrylamide solution 30% (37.5:1)

SDS-PAGE is used for the separation of proteins through electrophoresis and it is based on the fact that charged molecules will migrate through a matrix upon application of an electrical field. The matrix for protein electrophoresis separation is polyacrylamide. Acrylamide is a potentially dangerous chemical compound that is mainly used to synthesize polyacrylamide which in turn is used in gel electrophoresis (SDS-PAGE). Polyacrylamide is a commonly used electrophoresis matrix for size separation of proteins and nucleic acids. The gel matrix is formed by free radical polymerization of Acrylamide and a crosslinker (Bisacrylamide). N, N’-Methylenebisacrylamide is used as a reversible cross-linking reagent during the polymerization of polymers such as polyacrylamide. Acrylamide monomers polymerize into long chains by a reaction initiated by a free radical-generating system. These chains become cross-linked by N, N’- Methylenebisacrylamide and form a gel.