Gelatin Agar is recommended for cultivation and identification of Vibrio species. This medium is formulated in accordance with APHA for the cultivation and characterization of Vibrio species from foods and faeces. Clinical specimens must be obtained early in the disease as possible because the duration of excretion of the pathogen is short. V.cholerae appear transparent and usually have a characteristic cloudy zone around colony, which becomes more definiteafter few minutes of refrigeration. When these colonies are viewed in oblique light they appear iridescent green to bronze coloured and finely granular.
1.Bruno Gomez-Gil and Ana Roque, Isolation, Enumeration and Preservation of the Vibrionaceae, F.L. Thompson, B. Austinand J. Swings. The Biology of Vibrios, ASM Press.2.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C.