Soluble in about 1 part of water, practically insoluble in acetone and in alcohol
Solubility (IP)
Freely soluble in water, practically insoluble in ethanol (95%) in acetone, in chloroform and in ether
Identification A : TLC (IP, BP)
The principal spot in the chromatogram obtained with the test solution corresponds to that in the chromatogram obtained with reference solution (a). The test is not valid unless the chromatogram obtained with reference solution (b) shows three clearly separated spots.
Identification B : Melting point (IP, BP)
The melting point of the crystals, after washing with 20 ml of water and drying at 105°C, is about 235°C with decomposition
Identification C : (IP, BP)
A violet colour is produced
Identification D: Sulphates (IP, BP)
Complies
pH (1% in water at 25°C) : (IP, BP)
5.50-7.50
Specific optical rotation : (IP, BP)
+103.00 to +115.00° (1% in water at 20±0.5°C)
Kanamycin B : (IP, BP)
Any spot corresponding to kanamycin B in the chromatogram obtained with the test solution is not more intense than the spot in the chromatogram obtained with the reference solution
Sulfate (IP, BP)
23.00 - 26.00%
Sulfated ash (IP, BP)
< = 0.50%
Loss on drying (IP, BP)
<= 5.00% (at 60°C at a pressure not exceeding 0.7 kPa for 3 h)