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KCN HiVeg™ Broth Base w/o KCN
Potassium Cyanide HiVeg Broth Base with KCN supplementation is used for differentiation of the members of Enterobacteriaceae on the basis of Potassium Cyanide tolerance.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone No. 3 | 3.0 |
| Disodium phosphate | 5.64 |
| Monopotassium phosphate | 0.225 |
| Sodium chloride | 5.0 |
Final pH (at 25°C) 7.6 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 13.9 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in 100 ml amounts and sterlize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to room temperature and aseptically add sterile 1.5 ml of 0.5% Potassium Cyanide solution to each 100 ml of basal medium. Mix thoroughly and dispense in 1 ml amounts.
CAUTION: Being fatally toxic extreme care is needed while handling Potassium Cyanide solution. Never mouthpipette Potassium Cyanide solution.
Principle and Interpretation
Potassium Cyanide HiVeg Broth Base is prepared by using HiVeg peptone No.3 which is free of BSE/TSE risks. This medium is the modification of Potassium Cyanide Broth Base which was formulated by Moeller (1) and further modified by Edwards and Ewing (2) and Edwards and Fife (3) for differentiation of the members of Enterobacteriaceae. HiVeg peptone No.3 provides nitrogenous compounds, sulphur and trace elements essential for growth. Phosphates buffer the medium. Sodium chloride maintains the osmotic equilibrium. Potassium cyanide inhibits many bacteria including Salmonella, Shigella and Escherichia while members of the group Klebsiella, Citrobacter, Proteus grow well. Potassium cyanide medium usually remains stable upto 4 weeks at 4°C (3). Elevated temperature leads to accelerated deterioration of KCN in the medium or evaporation of cyanide (4). The KCN should be destroyed before autoclaving by the addition of a crystal of ferric sulphate and 0.1 ml of 40% potassium hydroxide per tube (5).
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Technical Data
Product Profile :
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV936 | M936 |
| HiVeg peptone No. 3 | Proteose peptone |
Recommended for : Differentiation of the members of Enterobacteriaceae on the basis of potassium cyanide tolerance.
Reconstitution : 13.9 g/l
Quantity on preparation (500g): 35.97 L
pH (25°C) : 7.6 ± 0.2
Supplement : 0.5% Potassium Cyanide Solution
Sterilization : 121°C / 15 minutes.
Storage : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Colour and Clarity
Light amber coloured, clear solution without any precipitate.
Reaction
Reaction of 1.39% w/v aqueous solution is pH 7.6 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours with added sterile 0.5% Potassium Cyanide Solution.
| Organisms (ATCC) | Growth |
|---|---|
| Citrobacter freundii (8090) | luxuriant |
| Escherichia coli (25922) | inhibited |
| Klebsiella pneumoniae (13883) | luxuriant |
| Proteus vulgaris (13315) | luxuriant |
| Pseudomonas aeruginosa (27853) | luxuriant |
| Salmonella serotype Enteritidis (13076) | inhibited |
| Shigella flexneri (12022) | inhibited |
References
- Moeller V., 1954, Acta. Pathol. Microbiol. Scand., 34:115.
- Edwards P.R. and Ewing W.H., 1955, Minneapolis, Burgess Publishing Co.
- Edwards P.R. and Fife M.A., 1956, Appl. Microbiol., 4:46.
- Munson T.E., 1974, Appl. Microbiol., 27:262.
- Cowan S.T. and Steel K.J., 1966, Manual for the Identification of Medical Bac- teria, Cambridge, Cambridge University Press.
| Product Name | Potassium Cyanide HiVeg™ Broth Base w/o KCN |
|---|---|
| SKU | MV936 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Collee J.G., Fraser A.G., Marmion B. P., Simmons A., (Eds.), Mackie and McCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone |
| Customized Product Available | No |
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Potassium Cyanide Broth Base w/o KCN
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