Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for selective enrichment of Listeria species.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate No. 1 | 10.0 |
| Yeast extract | 5.0 |
| HiVeg extract | 5.0 |
| Sodium chloride | 20.0 |
| Disodium hydrogen phosphate | 9.6 |
| Monopotassium hydrogen phosphate | 1.35 |
| Esculin | 1.0 |
| Nalidixic acid | 0.02 |
| Acriflavin hydrochloride (Trypaflavin) | 0.012 |
Final pH (at 25°C) 7.4 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 52 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Listeria Enrichment HiVeg Broth, Modified is prepared by completely replacing animal based peptones with vegetable peptones which make the medium free of BSE/TSE risks. Listeria Enrichment HiVeg Broth, Modified is used for selective enrichment of Listeria species from milk, milk products and other foods.
This medium contains HiVeg hydrolysate No.1, yeast extract and HiVeg extract which provide essential nutrients like carbon and nitrogenous compounds including vitamins, amino acids and trace ingredients. Phosphates provide buffering action to the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and Acriflavin inhibits the growth of gram-negative and gram-positive organisms respectively (1, 2, 3) except Listeria species. For the enrichment, 25 g or 25 ml sample is added to 225 ml medium in a stomacher bag. Homogenize the material if required. Incubation is carried out at 30°C for upto 7 days and the sample is subcultured on Listeria Selective HiVeg Agar (MV567) after 1, 2 and 7 days.
Product Specifications
Product Base
- Vegetable based: HiVeg hydrolysate No. 1, HiVeg extract
- Animal based: Tryptose, Beef extract
- Recommended for
- Selective enrichment of Listeria species.
- Reconstitution
- 52.0 g/l
- Quantity on preparation (500g)
- 9.61 L
- Quantity on preparation (100g)
- 1.92 L
- Supplement
- None
- Sterilization
- 121°C / 15 minutes.
- Storage
- Dry Medium and Prepared Medium 2 - 8°C.
Quality Control
Appearance of powder
Light yellow coloured may have slight greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Yellow coloured, clear to slightly opalescent solution with a bluish tinge.
Reaction
Reaction of 5.2% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli (25922) | 2 X 103-104 | inhibited |
| Listeria monocytogenes (19111) | 102-103 | luxuriant |
| Listeria monocytogenes (19112) | 102-103 | luxuriant |
| Listeria monocytogenes (19117) | 102-103 | luxuriant |
| Listeria monocytogenes (19118) | 102-103 | luxuriant |
| Staphylococcus aureus (25923) | 2 X 103-104 | inhibited |
References
- Lovette J., Francis D.W. and Hunt J.M., 1987, J. Food Prot., 50:188
- Lee W.K. and McClain D., 1986, Appl. Environ. Microbiol., 52:1215
- McClain D. and Lee W.H., 1988, J. Assoc. Off. Anal. Chem., 71:660.
| Product Name | Listeria Enrichment HiVeg™ Broth, Modified |
|---|---|
| SKU | MV888 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. |
| Customized Product Available | No |
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