Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Recommended for cultivation of organisms from body fluids and other materials containing antibacterials such as Penicillin, Streptomycin and Sulphonamides.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ peptone No.3 | 10.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 1.000 |
| Sodium chloride | 5.000 |
| Thiol compound | 8.000 |
| p-Amino benzoic acid (PABA) | 0.050 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 29.05 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks to a depth of 6 cm for neutralization of Penicillin or in shallow layers for neutralization of Streptomycin. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Thiol Medium is used for culturing microorganisms from body fluids and also other materials containing antibiotics like penicillin, streptomycin or sulphonamides. The efficacy of Thiol Medium to retain viability of Vibrio was initially described by Huddleson (1). The ability of Thiol Medium to neutralize antibacterials was demonstrated by Christensen (2).
This media can also be used for the cultivation and maintenance of Haemophilus, Vibrio and Meningococci (1). Thiol Broth which is Thiol Medium devoid of agar is also recommended for growing anaerobic bacteria in blood cultures and for recovery of nutritionally variant Streptococci (3,4) and Bacteriodes (5,6). Thiol HiVeg™ Broth is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones.
HiVeg™ peptone No.3 and yeast extract provide nitrogenous compounds, vitamin B complex and other essential growth nutrients. Dextrose is the energy source. p-Amino benzoic acid serves as a preservative.
Type of specimen
Please add specimens
Specimen Collection and Handling:
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent solution.
Reaction
Reaction of 2.9% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH
6.90-7.30
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours. Growth observed after addition of antibiotic concentrations upto 100 units of Penicillin or 1,000 micrograms of Streptomycin.
| Organism | Inoculum (CFU) |
Growth |
|---|---|---|
| Neisseria meningitidis ATCC 13090 | 50-100 | poor-fair |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | good-luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Thiol HiVeg™ Broth |
|---|---|
| SKU | MV853 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Huddleson I. F., 1948, J. Bacteriol., 56:508. |
| Customized Product Available | No |
Write Your Own Review
Thiol Broth
As low as
₹5,882.00