Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Recommended for the selective isolation and enumeration of Yersinia enterocolitica from various samples.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ special peptone | 20.000 |
| Yeast extract | 2.000 |
| Mannitol | 20.000 |
| Sodium pyruvate | 2.000 |
| Sodium chloride | 1.000 |
| Magnesium sulphate | 0.010 |
| Synthetic detergent No.III | 0.500 |
| Neutral red | 0.030 |
| Crystal violet | 0.001 |
| Agar | 12.500 |
| Final pH (at 25°C) | 7.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 29.02 grams in 500 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add reconstituted contents of 1 vial CTN Selective Supplement(FD034). Mix well before pouring into sterile Petri plates.
Principle And Interpretation
Yersinia enterocolitica is widely distributed in lakes and reservoirs. Epizootic outbreaks of diarrhea,lymphadenopathy, pneumonia and spontaneous abortions occur in various animals. It is the most common species of Yersinia recovered from specimens. Y. enterocolitica is biochemically more active at room temperature than at 37°C.
This medium is prepared by completely replacing animal based peptones with vegetable peptones to avoid BSE/TSE risks associated with animal peptones. It is used to isolate Y. enterocolitica from specimens. The formulation of animal based media is based on CIN Agar of Schiemann (1,2) and is recommended by ISO Committee (3). Schiemann (4) modified his previous formula of CIN medium by replacing bile salts with sodium deoxycholate. In this medium bile salts are replaced by synthetic detergent No. III. The medium differentiates between mannitol fermenting and non-fermenting bacteria. Microorganisms that ferment the sugar mannitol acidify the medium and cause a localized drop in pH around the colonies. In presence of neutral red, the colonies take red colour. Mannitol negative organisms form colorless and translucent colonies. The medium is selective due to the presence of Synthetic detergent No. III and crystal violet, which inhibit gram-positive and a number of gram-negative bacteria. Addition of antibiotic supplement makes it highly selective for Yersinia.
Typical colonies of Y. enterocolitica will form dark red colonies resembling bulls eye, which are normally surrounded by a transparent border. Colony size, smoothness and ratio of the border to center diameter may vary among different serotypes. For the isolation of Y. enterocolitica by direct plating and pour plating, inoculate the specimen directly onto the medium. Incubate at 22-32°C for 24-48 hours or suspend the sample (food, faeces, etc.) in sterile Phosphate Buffer Saline and incubate for upto 21 days (8) at 4°C. Periodically subculture samples onto Agar Plate and incubate as above.
Type of Specimen
Food and dairy samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (5,6,7).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Serratia liquefaciens, Citrobacter freundi and Enterobacter agglomerans may resemble Y.enterocolitica that can be further identified by biochemical tests.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within theexpiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm, comparable with 1.25% Agar gel.
Colour and Clarity of prepared medium
Orange red coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 5.8% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed with added CTN Selective Supplement (FD034) after an incubation at 22-32°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Enterococcus faecalis ATCC 29212 (00087*) | >=104 | inhibited | 0% | |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% | |
| Proteus mirabilis ATCC 25933 | >=104 | inhibited | 0% | |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | >=104 | inhibited | 0% | |
| Yersinia enterocolitica ATCC 27729 | 50-100 | good-luxuriant | >=50% | transluscent with dark pink centre & bile precipitate. |
Key : (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).
| Product Name | Yersinia Selective HiVeg™ Agar Base |
|---|---|
| SKU | MV843 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Schiemann D. A., 1979, Can. J. Microbiol., 25: 1298. |
| Customized Product Available | No |
Write Your Own Review
Yersinia Isolation Agar
As low as
₹6,815.00
Yersinia Selective Agar Base, w/ 1.2% Agar
As low as
₹7,487.00
CTN Selective Supplement
As low as
₹1,717.00
Upsell Products
Yersinia Selective Agar Plate
As low as
₹1,227.00