Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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General Information
Pfizer Selective Enterococcus HiVeg Agar is used for selective isolation and cultivation of Enterococci.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV787 | M787 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
| Synthetic detergent | Bile salts |
Recommended for : Selective isolation and cultivation of Enterococci.
Reconstitution : 58.0 g/l
Quantity on preparation (500g): 8.62 L
pH (25°C) : 7.1 ± 0.2
Supplement : None
Sterilization : 121°C / 15 minutes.
Storage : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Composition ** :
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 21.0 |
| HiVeg peptone | 6.0 |
| Yeast extract | 5.0 |
| Synthetic detergent | 3.0 |
| Sodium chloride | 5.0 |
| Sodium citrate | 1.0 |
| Esculin | 1.0 |
| Ferric ammonium citrate | 0.5 |
| Sodium azide | 0.25 |
| Agar | 15.0 |
Final pH (at 25°C) 7.1 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions :
Suspend 58 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Warning: Sodium Azide has a tendency to form explosive metal azides with plumbing materials. It is advisable to use enough water to flush off the disposables.
Principle and Interpretation :
Pfizer Selective Enterococcus HiVeg Agar is prepared by using vegetable peptones in place of animal based peptones which makes the medium free of BSE/TSE risk. Pfizer Selective Enterococcus HiVeg Agar is used for selective isolation and cultivation of Enterococci. The importance of esculin hydrolysis in identifying Enterococci was first noted by Rochaix as other Streptococci fail to do so (1). Isenberg used sodium azide in the medium to inhibit gram negative organisms (2).
HiVeg hydrolysate, HiVeg peptone and yeast extract provide the nutrients like nitrogenous compounds, carbon, sulphur, vitamin B complex and trace ingredients for the growth of Enterococci. Esculin, a glycoside is hydrolyzed by Enterococci to esculetin and dextrose. Esculetin reacts with ferric ammonium citrate to form a dark brown to black coloured complex (3). Synthetic detergent and sodium azide inhibit gram-positive (except Enterococci) and gram-negative bacteria respectively.
Quality Control :
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Light amber coloured, clear to slightly opalescent gel with a bluish tinge forms in petri plates.
Reaction
Reaction of 5.8% w/v aqueous solution is pH 7.1 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Esculin Hydrolysis |
|---|---|---|---|
| Enterobacter aerogenes (13048) | 10²-10³ | inhibited | - |
| Enterococcus faecalis (29212) | 10²-10³ | luxuriant | + |
| Escherichia coli (25922) | 10²-10³ | inhibited | - |
| Staphylococcus aureus (25923) | 10²-10³ | fair-good | - |
Key : + = blackening around the colony.
MV787 Pfizer Selective Enterococcus HiVeg Agar
1. Control
2. Enterococcus faecalis
References :
- Rochaix, 1924, C.R. Soc. Biol., 90 : 771.
- Isenberg, Goldberg and Sampson, 1970, Appl. Microbiol., 20 : 433.
- MacFaddin J.F., 2000 (ed), Biochemical Tests for Identification of Medical Bacteria, 3rd edition, Lippincott Williams and Wilkins, New York
| Product Name | Pfizer Selective Enterococcus HiVeg™ Agar |
|---|---|
| SKU | MV787 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Belzer R., Vergleichende Untersuchungen von Enterokokkenselektivnährböden. Inaug. Dissert., Univ. München, 1983. |
| Customized Product Available | No |
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