Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for selective isolation and identification of Salmonellae from various specimens.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ peptone | 8.625 |
| Yeast extract | 3.000 |
| HiVeg™ extract No. 1 | 6.000 |
| Lactose | 11.500 |
| Sucrose | 13.000 |
| Synthetic detergent no. 1 | 2.000 |
| Trisodium citrate, pentahydrate | 9.300 |
| Sodium thiosulphate pentahydrate | 4.250 |
| L-Phenylalanine | 5.000 |
| Disodium hydrogen phosphate dihydrate | 1.000 |
| Ferric citrate | 0.500 |
| Magnesium sulphate | 0.400 |
| Brilliant green | 0.00166 |
| Neutral red | 0.022 |
| Aniline blue | 0.250 |
| Metachrome yellow | 0.470 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.1±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 76.15 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Salmonella and Shigella are gram-negative, facultatively anaerobic, non-sporulating rods in the family Enterobacteriaceae. They are widely distributed in animals. Salmonella Agar, ONOZ was developed by öNöZ (4) for rapid detection of Salmonella and Shigella species from clinical specimens.
Salmonella HiVeg™ Agar, ONOZ is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg™ peptone, yeast extract and HiVeg™ extract No. 1 provide nitrogenous compounds, vitamin B complex and other essential growth nutrients. Lactose and sucrose are the fermentable carbohydrates. Synthetic detergent no. 1, brilliant green and sodium citrate inhibit gram-positive organisms. Sodium thiosulphate and ferric citrate enable the detection of hydrogen sulphide production indicated by colonies with black centers. Lactose and sucrose fermenting members of Enterobacteriaceae are partially inhibited, and their colonies can be differentiated by means of the colour produced in the presence of the indicators -neutral red and aniline blue.
Proteus species deaminate phenylalanine to give phenylpyruvate, which forms a dark brown complex with iron ions. Phenylalanine also neutralizes chloramphenicol, which aids in the detection of Salmonella from patients under treatment.
Type of specimen
Clinical: Faeces, Urine; Water samples and Food samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (2). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- The medium is selective for Salmonella and may not support the growth of other microorganisms.
- Due to nutritional variations, some strains may show poor growth.
- Final confirmation of suspected colonies must be carried out by serological and biochemical tests.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Beige to light brown homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Greenish brown coloured clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 8.03% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH: 6.90-7.30
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony | Colour change of medium |
|---|---|---|---|---|---|
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | good-luxuriant | >=50% | bluish or yellowish | yellow |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good | 40-50% | blue with bile precipitation | blue |
| Klebsiella pneumoniae ATCC 13883 (00097*) | 50-100 | poor-fair | 20-30% | bluish-purple, may have slight precipitation ring around colony | bluish green |
| Proteus mirabilis ATCC 25933 | 50-100 | good-luxuriant | >=50% | dark brown to black | dark yellow |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | good-luxuriant | >=50% | yellow to brown | yellow |
| Salmonella Typhi ATCC 6539 | 50-100 | good-luxuriant | >=50% | yellow with or without black centres | yellow |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | good-luxuriant | >=50% | yellow with black centres | yellow |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | good-luxuriant | >=50% | yellow to brown | dark brown |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% |
Key: (*) Corresponding WDCM numbers. (#) Formerly known as Enterobacter aerogenes
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Salmonella HiVeg™ Agar, ONOZ |
|---|---|
| SKU | MV573 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. öNöZ E., Hoffmann K., 1978, Zbl. Bakt. Hyg., I. Abt. Orig., A240:16. |
| Customized Product Available | No |
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