Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for selective enrichment and cultivation of Streptococci.
Composition
| Ingredients | Gms / Litre |
|---|---|
| HiVeg™ hydrolysate | 10.000 |
| HiVeg™ hydrolysate No. 1 | 10.000 |
| Yeast extract | 10.000 |
| Dextrose (Glucose) | 0.200 |
| Sodium azide | 0.065 |
| Crystal violet | 0.002 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.26 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense in 100 ml amounts in flasks. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 5%v/v sterile defibrinated rabbit blood. Mix well and dispense aseptically in 2 ml amounts in sterile tubes.
Principle And Interpretation
Streptococcus is a genus of spherical, gram-positive bacteria having both pathogenic and commensal role. Streptococci are subdivided into groups by antibodies that recognize surface antigens. These groups may include one or more species. The most important groupable streptococci are A, B and D. Individual species of Streptococcus are classified primarily based on their hemolytic properties (breakdown of red blood cells in a laboratory). Pike Streptococcal Broth is prepared as per the formula described by Pike (1) for selective enrichment and cultivation of haemolytic streptococci from throat swabs (2). This medium is also used to preserve Streptococcus pyogenes, Pneumococci and Haemophillus influenzae from nose and throat swabs (3). Pike Streptococcal HiVeg™ Broth Base is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg™ hydrolysate, HiVeg™ hydrolysate No. 1 and yeast extract provide nitrogenous nutrients, carbon, sulphur, vitamin B complex, trace elements for the growth of haemolytic Streptococci. Dextrose acts as the energy source. Crystal violet inhibits gram-positive bacteria and sodium azide inhibits gram-negative rods and non-haemolytic Streptococci (4).
Type of specimen
Please add specimens
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Yellow coloured clear solution without any precipitate
Reaction
Reaction of 3.0% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited |
| # Klebsiella aerogenes ATCC 13048 (00175*) | >=104 | inhibited |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | good-luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Pike Streptococcal HiVeg™ Broth Base |
|---|---|
| SKU | MV519 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Pike R. M., 1944, Proc. Soc. Exptl. Biol. and Med., 57:186. 2.Pike R. M., 1945, Am. J. Hygiene, 41:2 3.Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and McCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone 4.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore |
| Customized Product Available | No |
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