Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for antimicrobial susceptibility tests.
Composition
| Ingredients | Gms / Litre |
|---|---|
| HiVeg™ hydrolysate | 11.000 |
| HiVeg™ peptone | 3.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 3.000 |
| Starch, soluble | 1.000 |
| Disodium hydrogen phosphate | 2.000 |
| Sodium acetate | 1.000 |
| Magnesium glycerophosphate | 0.200 |
| Calcium gluconate | 0.100 |
| Cobaltous sulphate | 0.001 |
| Cupric sulphate | 0.001 |
| Ferrous sulphate | 0.001 |
| Zinc sulphate | 0.001 |
| Manganous chloride | 0.002 |
| Menadione | 0.001 |
| Cyanocobalamin | 0.001 |
| L-Cysteine hydrochloride | 0.020 |
| L-Tryptophan | 0.020 |
| Pyridoxine hydrochloride | 0.003 |
| Calcium pantothenate | 0.003 |
| Nicotinamide | 0.003 |
| Biotin | 0.0003 |
| Thiamine hydrochloride | 0.00004 |
| Adenine | 0.010 |
| Guanine | 0.010 |
| Xanthine | 0.010 |
| Uracil | 0.010 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 23.4 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
The goal of an antimicrobial susceptibility test is to predict through an in vitro assessment the likelihood of successfully treating an infection with a particular antimicrobial agent. There are several continual or novel methods for performing antibacterial susceptibility testing. These include the disk diffusion test, broth microdilution, agar gradient and rapid automated instrument methods (1). Hi-Sensitivity™ Test HiVeg™ Broth, which is used for antimicrobial susceptibility tests, is a semi-defined medium in which the mineral contents have been stabilized to give reproducible results. The thiamine and thymidine content is very low thus making it most suitable for testing antimicrobial activity of sulphonamides. However some mutant strains which are totally dependent on thiamine and thymidine for their growth, will not grow in Hi-Sensitivity™ Test HiVeg™ Broth, due to very low levels of these compounds in the media as they are the naturally occurring antagonist of trimethoprim. These strains should be carefully recognized (2,3,4).
Hi-Sensitivity™ Test Broth has been so designed to overcome the problems occurring in Mueller-Hinton Media that are as follows (5,6,7,8,9,10,11).
- Mueller Hinton Agar and Mueller Hinton Broth give different MIC values.
- Mueller Hinton Agar shows antagonistic effect towards tetracycline.
- High levels of sulphonamide and trimethoprim antagonists.
- Media prepared using peptone of different manufacturers give poor reproducibility.
- Poor growth supporting ability for Streptococci and variable growth rates with gram-positive organisms.
Some pathogenic organisms are nutritionally dependent due to their intrinsic demands for special growth factors. Hi-Sensitivity™ Test HiVeg™ Broth is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg™ hydrolysate, HiVeg™ peptone, dextrose, and vitamins provides nitrogen, carbon compounds and other essential growth nutrients.
Type of specimen
Isolated Microorganism from Clinical samples.
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets
Limitations
- Some pathogenic organisms are nutritionally dependent due to intrinsic demands for special growth factors
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Basal medium: Light yellow; After addition of 5%v/v laked blood : Red to chocolate coloured, Basal medium :clear to slightly opalescent; After Addition: opalescent solution in tubes
Reaction
Reaction of 2.34% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | good-luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | good-luxuriant |
| ** Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | good-luxuriant |
| $ Phocaeicola vulgatus ATCC 8482 | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers **Formerly known as Bacillus subtilis subsp. spizizenii $ Formerly known as Bacteroides vulgatus
Storage and Shelf Life
Store dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (12.13).
| Product Name | Hi-Sensitivity™ Test HiVeg™ Broth |
|---|---|
| SKU | MV486 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.). 2003, Manual of Clinical Microbiology,8th Ed.,American Society for Microbiology, Washington, D.C. 2.Tanner E. I. and Bullin C. H., 1974, J. Clin. Path., 27:565. 3.Thomas M. and Bond L., 1973, Med. Lab. Technol., 30:277. 4.Barker J., Healing D., and Hutchinson J. G. P., 1972, J. Clin. Path., 25:10865.Ericsson H. M. and Sherris J. C., 1971, Acta. Pathol. Microbiol Scand Suppl., 217:1. 6.Garrod L. P. and Waterworth P. M., 1971, J. Clin. Path., 24:779. 7.Reller L. B., Schoenknecbt F. D., Kenny M. A. and Sherris J. C., 1974, J. Infect. Dis., 130:454. 8.Duncan I. B. R., 1974, Antimicrob. Agents Chemother., 5:9.9.Yourassowsky E., Vanderlinden M. P. and Schoutens E., 1974, J. Clin. Path., 27:897.10.Neussil H., 1976, Chemother., Vol. 2 : 33. 11.Bridson E.Y., 1976, Arztl. Lab., 22:373. |
| Customized Product Available | No |
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