Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Enrichment of Staphylococcus aureus and also for disinfectant testing.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 10.0 |
| HiVeg infusion | 5.0 |
| Sodium chloride | 5.0 |
| Final pH (at 25°C) | 6.8 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 20 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by using HiVeg peptone and HiVeg infusion which is BSE/TSE risks free. This medium is the modification of Staphylococcus Aureus Enrichment Broth which is a selective medium used in the membrane filter technique for isolating pathogenic Staphylococci e.g. Staphylococcus aureus. Staphylococcus aureus is coagulase positive and is well documented as a human opportunistic pathogen and has been major cause of morbidity and mortality(1). Staphylococcus Aureus Enrichment HiVeg Broth, like the conventional medium is also used for disinfectant testing.
The medium contains ingredients like HiVeg infusion and HiVeg peptone, which provide necessary nutrients to the growth of Staphylococcus aureus when used as test organism in disinfectant testing. Sodium chloride in the medium heps to maintain osmotic equilibrium.
Product Details
Vegetable based (Code MV)
- MV464
- HiVeg peptone
- HiVeg infusion
Animal based (Code M)
- M464
- Peptic digest of animal tissue
- Meat (Beef) infusion
Reconstitution Parameters
| Reconstitution | 20.0 g/l |
| Quantity on preparation (500g) | 25.0 L |
| pH (25°C) | 6.8 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Yellow coloured, clear solution without any precipitate.
Reaction
Reaction of 2.0% w/v aqueous solution is pH 6.8 ± 0.2 at 25°C
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Staphylococcus aureus (25923) | 102-103 | luxuriant |
| Staphylococcus aureus (6538) | 102-103 | luxuriant |
Storage and Shelf Life
Dry Medium Below 30°C, Prepared Medium 2-8°C.
| Product Name | Disinfectant Test HiVeg™ Broth (Staphylococcus aureus Enrichment HiVeg™ Broth) |
|---|---|
| SKU | MV464 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Easmon C. S. F., Adlam C., Staphylococci and staphylococcal infections. Vol. I & II, 1983, Academic Press, London 2.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition. 3.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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