Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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MIO HiVeg™ Medium (Motility Indole Ornithine HiVeg™ Medium)
For the identification of Enterobacteriaceae on the basis of motility, indole production and ornithine decarboxylase activity.
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Intended Use
Motility Indole Ornithine HiVeg Medium (MIO HIVeg Medium) is used for the identification of Enterobacteriaceae on the basis of motility, indole production and ornithine decarboxylase activity. It is recommended for identification of Enterobacteriaceae on the basis of motility, indole production and ornithine decarboxylase activity.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV378 | M378 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 10.0 |
| HiVeg peptone | 10.0 |
| Yeast extract | 3.0 |
| L-Ornithine hydrochloride | 5.0 |
| Dextrose | 1.0 |
| Bromo cresol purple | 0.02 |
| Agar | 2.0 |
Final pH (at 25°C) 6.5 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 31 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in test tubes in 5 ml amounts. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes in an upright position.
Reconstitution and Storage
| Reconstitution | 31.0 g/l |
|---|---|
| Quantity on preparation (500g) | 16.12 L |
| pH (25°C) | 6.5 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
| Storage | Dry Medium - Below 30°C. Prepared medium 2 - 8°C. |
Principle and Interpretation
MIO HiVeg Medium is prepared by using vegetable peptones which are free of BSE/TSE risks. MIO HiVeg Medium is the modification of MIO Medium which was formulated by Ederer and Clark (1) and Oberhofer and Hajkowski (2) for detection of motility, indole and ornithine decarboxylation in single culture tube.
HiVeg hydrolysate and HiVeg peptone provide amino acids and other nitrogenous substances. Yeast extract is the source of vitamin B complex. Dextrose is the fermentable carbohydrate. Cultures are stab-inoculated.
Motility and Ornithine decarboxylation reactions are read before testing indole production. Motile organisms show either diffused growth or turbidity extending away from stab inoculation line while non-motile organisms grow along the stab-line. Organisms ferment dextrose to form acid which causes the pH indicator bromo cresol purple to change from purple to yellow. Organisms possessing ornithine decarboxylase, decarboxylates ornithine to putrescine which increases the pH making it alkaline, indicated by colour change from yellow to purple throughout the medium. Decarboxylase negative reaction is indicated by yellow colour or yellow with a purple band near the top of the medium. Indole is produced from tryptophan present in HiVeg hydrolysate. The indole produced combines with the aldehyde present in the Kovac's reagent to form a red complex.
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Semisolid, comparable with 0.2% Agar gel.
Colour and Clarity
Purple coloured, clear to slightly opalescent gel forms in tubes as butts.
Reaction
Reaction of 3.1% w/v aqueous solution is pH 6.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 40 - 48 hours.
| Organisms (ATCC) | Inoculum | Growth (CFU) | Motility | Indole | Ornithine** |
|---|---|---|---|---|---|
| Enterobacter aerogenes (13048) | 102-103 | luxuriant | + | - | + |
| Escherichia coli (25922) | 102-103 | luxuriant | + | + | + |
| Klebsiella pneumoniae (13883) | 102-103 | luxuriant | - | - | - |
| Proteus mirabilis (25933)* | 102-103 | luxuriant | + | - | + |
Key :
- + = positive reaction
- - = negative reaction
- * = motility of Proteus mirabilis is temperature dependent. It is more pronounced at 20-22°C and almost absent at 35-37°C.
- ** = Decarboxylation
MV378 MIO HiVeg Medium :
- Control
- Escherichia coli
- Klebsiella pneumoniae
- Proteus mirabilis
| Product Name | MIO HiVeg™ Medium (Motility Indole Ornithine HiVeg™ Medium) |
|---|---|
| SKU | MV378 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. MacFaddin J. F., 2000, Biochemical tests for Identification of Medical Bacteria, 3rd Ed., Lippincott, Williams and Wilkins,Baltimore. 2.Ederer G. M. and Clark M., 1970, Appl. Microbiol., 20:849. 3.Oberhofer J. R. and Hajkowski R., 1970, Am. J. Clin. Pathol., 54:726. 4.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore. 5.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,Inc., New York. |
| Customized Product Available | No |
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