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Lysine Lactose HiVeg™ Broth
For determination of lysine decarboxylase activity of lactose non-fermenting members of Enterobacteriaceae , especially Salmonellae.
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Intended Use
Lysine Lactose HiVeg Broth is used for determination of lysine decarboxylase activity of lactose non-fermenting members of Enterobacteriaceae especially Salmonellae.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | ||
|---|---|---|---|
| MV330 | HiVeg peptone No.2 | M330 | Pancreatic digest of gelatin |
Recommended for : Determination of lysine decarboxylase activity of lactose non-fermenting members of Enterobacteriaceae.
| Reconstitution | : | 24.0 g/l |
| Quantity on preparation (500g) | : | 20.83 L |
| pH (25°C) | : | 6.8 ± 0.2 |
| Supplement | : | None |
| Sterilization | : | 121°C / 15 minutes. |
| Storage | : | Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone No. 2 | 5.0 |
| Yeast extract | 3.0 |
| Dextrose | 1.0 |
| L-Lysine | 5.0 |
| Lactose | 10.0 |
| Bromo cresol purple | 0.02 |
Final pH (at 25°C) 6.8 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 24 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes in 5 ml amounts and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Lysine Lactose HiVeg Broth is prepared by using HiVeg peptone No. 2 in place of Pancreatic digest of gelatin which makes the medium free of BSE/TSE risks. This medium is the modification of medium formulated by Falkow (1) for detection of lysine decarboxylase by means of a colour reaction in enteric bacilli.
HiVeg peptone No.2 and yeast extract provide nitrogenous and carbonaceous nutrients. Dextrose and lactose are the fermentable sugars. L-Lysine is the substrate which is decarboxylated due to decarboxylase enzyme activity. Bromo cresol purple acts as pH indicator.
The enteric bacilli produce acid in an initial fermentation. The lactose non-fermenters produce acid from dextrose resulting in yellow colour. L-Lysine is decarboxylated to form cadaverine resulting in an alkaline reaction and the broth reverts to purple colour.
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Purple coloured, clear solution without any precipitate.
Reaction
Reaction of 2.4% w/v aqueous solution is pH 6.8 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C after 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Colour of medium | Lactose Fermentation | Lysine decarboxylation |
|---|---|---|---|---|
| Escherichia coli (25922) | 102-103 | yellow | + | - |
| Proteus vulgaris (13315) | 102-103 | greenish yellow | - | (+) |
| Salmonella serotype Typhimurium (14028) | 102-103 | purple | - | + |
| Salmonella serotype Enteritidis (13076) | 102-103 | purple | - | + |
| Serratia marcescens (8100) | 102-103 | purple | - | + |
Key : + = positive reaction
- = negative reaction
(+) = delayed positive reaction
References
- Falkow A., 1958, J. Clin. Path., 29:598.
- Ewing, Davis and Reavis, 1959, U.S. Dept.H.E.W., C.D.C., Atlanta.
- Ewing and Johnson, 1960, Internat. Bull. Bact. Nomen. and Tax., 10:223.
- Edwards and Ewing, 1962, Burgess Publ. Co., Minneapolis, Minn.
| Product Name | Lysine Lactose HiVeg™ Broth |
|---|---|
| SKU | MV330 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Falkow A., 1958, J. Clin. Pathol., 29:598 |
| Customized Product Available | No |
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