Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Sulphite HiVeg Agar is used for detection of thermophilic sulphide producing anaerobic microorganisms.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 10.0 |
| Sodium sulphite | 1.0 |
| Agar | 20.0 |
Final pH (at 25°C) 7.6 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 31 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in screw capped tubes containing a clean iron nail in 15 ml amounts and cap the tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. As an alternate to iron nail, 10 ml of 5% ferric citrate solution may be used per litre of the medium.
Principle and Interpretation
Sulphite HiVeg Agar is developed by using HiVeg hydrolysate which is free from BSE/TSE risks associated with animal based peptones. Sulphite HiVeg Agar is the modification of Sulphite Agar which is prepared according to the formula described by Clark and Tanner (1) and is recommended by APHA (2) for detecting the thermophilic hydrogen sulphide producing anaerobic microorganisms. HiVeg hydrolysate provides nitrogenous compounds. Sodium sulphite is reduced and thus contribute in H₂S production by the thermophilic anaerobic bacteria. Agar acts as solidifying agent. Iron nails or feric citrate combines with dissolved oxygen in the medium and provides anaerobic conditions. Incubation at high temperature (55°C) favours growth of thermophilic organisms. Sulphite reduction is indicated by blackening of medium.
Technical Data
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV311 HiVeg hydrolysate |
M311 Casein enzymic hydrolysate |
Recommended for : Detection of thermophilic sulphide producing anaerobic microorganisms.
Reconstitution : 31.0 g/l
Quantity on preparation (500g) : 16.12 L
pH (25°C) : 7.6 ± 0.2
Supplement : Iron nail / 5% Ferric citrate solution.
Sterilization : 121°C / 15 minutes.
Storage : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Gelling
Firm, comparable with 2.0% Agar gel.
Colour and Clarity
Light amber coloured, clear to slightly opalescent gel forms in tubes.
Reaction
Reaction of 3.1% w/v aqueous solution is pH 7.6 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 55±2°C for 18 - 48 hours under anaerobic conditions.
Organisms (ATCC)
| Organisms (ATCC) | Inoculum (CFU) | Growth | Sulphide reduction |
|---|---|---|---|
| Bacillus stearothermophilus (10149) | 30-100 | good | - |
| Clostridium thermosaccharolyticum (7956) | 30-100 | good | + |
| Desulfotomaculum nigrificans (19858) | 30-100 | good | + |
Quality Control
Appearance of powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
| Product Name | Sulphite HiVeg™ Agar |
|---|---|
| SKU | MV311 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Clark F. M. and Tanner F. W., 1937, Food Research, 2:27. 2.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,American Public Health Association, Washington, D.C. 3.Horwitz W., (Ed.), 2000, Official Methods of Analysis of AOAC International, 17th Ed., AOAC International, Gaithersburg,Md. |
| Customized Product Available | No |
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