Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for selective isolation and cultivation of Streptococci including group A B- haemolytic strains.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 15.000 |
| Soya peptone | 5.000 |
| Sodium chloride | 4.000 |
| Sodium citrate | 1.000 |
| L-Cystine | 0.200 |
| Sodium sulphite | 0.200 |
| Dextrose (Glucose) | 5.000 |
| Sodium azide | 0.200 |
| Crystal violet | 0.0002 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.6 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Mix well and dispense into sterile tubes or sterile flasks as desired. Autoclaving is not required if medium is used on the same day. If storage is desired, sterilize by autoclaving at A118°C for 15 minutes. Avoid overheating which causes the medium to become more inhibitory.
A Corresponds to 12lbs pressure
Principle And Interpretation
Streptococcus Selection Broth is based on the suggestion of Pike (1), for the selective isolation of Streptococci from various materials, specially those which are heavily contaminated with accompanying microbial flora (2). Welch et al (3) also reported the abilities of this medium to recover group A B-haemolytic Streptococci. Streptococcus Selection HiVeg™ Broth is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ hydrolysate, Soya peptone, dextrose and salts provide nutrients essential for the growth of Streptococci. Sodium azide, sodium sulphite inhibits gram-negative rods and the crystal violet suppresses Staphylococci. However, Streptococci are not affected by these inhibitors at these concentrations. Due to this reason, these media are useful in studies of streptococcal flora from nutritional, dental and epidemiological research. Growth of coliforms, Proteus, Pseudomonas and Bacillus species is markedly suppressed in this medium. However, some strains of Staphylococci and Pneumococci may grow in this medium.
Type of specimen
Water samples
Specimen Collection and Handling:
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(5) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- All presumptive streptococci must be confirmed for identification.
- Further biochemical and serological tests need to be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light to medium amber coloured clear to slightly opalescent solution in tubes
Reaction
Reaction of 3.06% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | >=104 | inhibited |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | none-poor |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | >=104 | inhibited |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | none-poor |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Streptococcus Selection HiVeg™ Broth |
|---|---|
| SKU | MV303 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Pike R.M., 1945, Am. J. Hyg., 41:2 2.Facklam and Carly, 1985, Manual of Clinical Microbiology, Lennette and others (Eds.), 4th ed., ASM, Washington D.C. 3.Welch D.F. et al, 1991, Am. J. Clin. Pathol., 95:587. |
| Customized Product Available | No |
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