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KF Streptococcal HiVeg™ Broth Base

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SKU:
MV249
For detection and enumeration of faecal Streptococci in water and for examination of faeces and other materials.

Intended Use

Recommended for detection and enumeration of faecal Streptococci in water samples and other materials.

Composition

Ingredients g/L
HiVeg™ special peptone 10.000
Yeast extract 10.000
Sodium chloride 5.000
Sodium ẞ-glycerophosphate 10.000
Sodium carbonate 0.636
Maltose 20.000
Lactose 1.000
Sodium azide 0.400
Phenol red 0.018
Final pH (at 25°C) 7.2±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 57.05 grams in 1000 ml of purified/distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. Cool to 45-50°C. If desired for use in the membrane filter technique, one ml of 1% Triphenyl Tetrazolium Chloride (TTC-FD057) may be added to each 100 ml of cooled broth. Mix well and dispense into sterile tubes or flasks as desired.

Principle And Interpretation

Streptococci are spherical, gram-positive bacteria and form a part of the normal commensal flora of the mouth, skin, intestine, upper respiratory tract of humans. Streptococci found in the faeces form the faecal Streptococci and constitute of Streptococci with group D Lancefield antigens. The types include Streptococcus faecalis, Streptococcus faecium, Streptococcus bovis and Streptococcus duran. They are low-grade pathogens and rarely cause disease. However, they may cause urinary tract infection in catheterized patients; mixed abdominal wound infections following gut surgery; and endocarditis on abnormal valves. Kenner-Faecal (KF) Medium were developed by Kenner et al (1,2) for detecting Streptococci in water and food materials. KF Streptococcal HiVeg™ Broth Base is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks.

HiVeg™ special peptone along with yeast extract provide nitrogen, carbon, sulphur, amino acids, vitamins and trace ingredients to the faecal Streptococci. Lactose and maltose are the fermentable carbohydrates and therefore serve as energy sources. Sodium azide is a selective agent, which hampers the growth of gram-negative bacteria.

2, 3, 5-Triphenyl Tetrazolium Chloride is reduced to insoluble formazan by actively metabolizing cells, resulting in the formation of pink or red colour. Bacteria resistant to azide, utilize lactose and / or maltose. Bacterial cells reduce TTC to insoluble formazan, resulting in the formation of pink to red colour.

Type of specimen

Water sample

Specimen Collection and Handling:

For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(3) After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions

Read the label before opening the container. The media should be handled by trained personnel only. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations :

  1. Due to nutritional variation, some strains may show poor growth.
  2. Further biochemical tests must be carried out for confirmation.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance
Light yellow to pinkish beige homogeneous free flowing powder

Colour and Clarity of prepared medium
Red coloured, clear solution without any precipitate

Reaction
Reaction of 5.7% w/v aqueous solution at 25°C. pH: 7.2±0.2 pH
7.00-7.40

Cultural Response

Cultural characteristics observed after an incubation at 35-37°C for 48-72 hours.

Organism Inoculum (CFU) Growth Colour of medium
Escherichia coli ATCC 25922 (00013*) >=104 inhibited
# Klebsiella aerogenes ATCC 13048 (00175*) >=104 inhibited
Enterococcus faecalis ATCC 29212 (00087*) 50-100 good-luxuriant yellow

Key: (*) Corresponding WDCM numbers. (#) Formerly known as Enterobacter aerogenes

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).

More Information
Product Name KF Streptococcal HiVeg™ Broth Base
SKU MV249
Product Type HiVeg™
Physical Form Powder
Origin Animal Free (Veg)
Packaging type HDPE
References 1.Kenner B. A., Clark H. F. and Kabler P. W., 1960, Am. J. Public Health, 50:1553.
2.Kenner B. A., Clark H. F. and Kabler P. W., 1961, Appl. Microbiol., 9:15.
Customized Product Available No
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