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Semisolid RV HiVeg™ Medium Base is used for the isolation of Salmonella from food stuffs and other materials based on selective motility.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV1428 | M1428 |
| HiVeg hydrolysate No. 1 | Tryptose |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
Recommended for: Isolation of Salmonella from food stuffs and other materials based on selective motility.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate No. 1 | 4.60 |
| HiVeg hydrolysate | 4.60 |
| Sodium chloride | 7.34 |
| Magnesium chloride, anhydrous | 10.93 |
| Malachite green | 0.037 |
| Agar | 2.70 |
Final pH (at 25°C) 5.4 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Reconstitution
Reconstitution: 30.20 g/l
Quantity on preparation (500g): 16.55 L
pH (25°C): 5.4 ± 0.2
Supplement: IMRV / RV Selective Supplement (FD193)
Sterilization
Boiling (DO NOT AUTOCLAVE)
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 15.10 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45°C and aseptically add 1 vial of IMRV/RV Selective Supplement (FD193). Mix well and pour into sterile petri plates.
Note: The motility of Salmonellas can be drastically reduced when the agar surface becomes too dry. Hence the plates should be well dried before use. If visible moisture occurs on the lid of the plates or the surface of agar, it must be removed. While incubation, incubate the plates aerobically in an upright position for no longer than 24 hours at 42°C.
Principle and Interpretation
Semisolid RV HiVeg Medium Base is prepared by using HiVeg hydrolysates which makes the medium free from BSE/TSE risks. Semisolid RV HiVeg Medium Base is the modification of Semisolid RV Medium Base based on the formulation described by DeSmedt et al (1) for the detection of motile Salmonella species from food and environmental specimens. This medium like the conventional medium detects more Salmonella positive samples than the routinely used enrichment procedures (2, 3, 4). HiVeg hydrolysate No.1, HiVeg hydrolysate provides the nitrogenous and carbonaceous substances and other essential growth nutrients. Novobiocin and malachite green in the medium inhibits most gram positive organisms. Salmonella survives at slight high osmotic pressure owing to presence of magnesium chloride in the medium, grows at slightly low pH and is comparatively resistant to malachite green.
The working of medium is based on the ability of Salmonella species to migrate in the selective medium competing with other motile organisms, thus producing opaque halos of growth. The motile bacteria will show a halo or zone of growth originating from inoculation spot.
Quality Control
Appearance of powder: Light green coloured, homogeneous, free flowing powder.
Gelling: Firm, comparable with 0.27% Agar gel.
Colour and Clarity: Blue coloured clear to slightly opalescent semisolid medium forms in petri plates.
Reaction: Reaction of 3.02% w/v aqueous solution is pH 5.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 42°C for 18-24 hours. when one drop of culture is inoculated in the centre of the medium plate.
Organisms (ATCC)
| Organisms (ATCC) | Inoculum (CFU) | Growth | Motility |
|---|---|---|---|
| Citrobacter freundii (8090) | 102-103 | inhibited | - |
| Pseudomonas aeruginosa (27853) | 102-103 | inhibited | - |
| Salmonella serotype Enteritidis (13076) | 102-103 | luxuriant | + # |
| Salmonella serotype Typhimurium (14028) | 102-103 | luxuriant | + # |
Key: # = opaque halos of growth originating from the inoculation spot
| Product Name | Semisolid RV HiVeg™ Medium Base |
|---|---|
| SKU | MV1428 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. De Smedt J.M., Balderdijk R., Rappold H. and Lautenschlaeger D., 1986, J. Food Prot., 49:510. |
| Customized Product Available | No |
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