Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Luria Bertani HiVeg™ Agar, Miller (Miller Luria Bertani HiVeg™ Agar)
Luria Bertani HiVeg Agar, Miller, is used for cultivation and maintenance of recombinant strains of
Escherichia coli for genetic and molecular studies and may be used for routine cultivation of not particularly fastidious
microorganisms
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Intended Use
Luria Bertani HiVeg Agar, Miller is used for cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular studies and may be used for routine cultivation of not particularly fastidious microorganisms.
Composition
| Ingredients | Gms/Litre |
|---|---|
| HiVeg hydrolysate | 10.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 10.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.5±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Luria Bertani HiVeg Agar, Miller is prepared by replacing casein enzymic hydrolysate with HiVeg hydrolysate which is free of BSE/TSE risks. It is the modification of Luria Bertani Agar formulated by Lennox (1) for cultivation and maintenance of recombinant strains of Escherichia coli. Luria Bertani HiVeg Agar, like the conventional media (2) are slightly different with double amount of sodium chloride. The medium is nutritionally rich for the growth of pure cultures of recombinant strains. Strains which are generally derived from Escherichia coli K12 are deficient in Vitamin B synthesis which are further modified by specific mutation to create auxotrophic strains and are unable to grow on nutritionally deficient media. HiVeg hydrolysate provides nitrogen and carbon while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for the membrane transport and maintains osmotic equilibrium of the medium.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Yellow to amber coloured, clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 4.0% w/v aqueous solution at 25°C. pH: 7.5±0.2
pH: 7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | >=70% |
| Escherichia coli DH5 alpha MTCC 1652 | 50-100 | luxuriant | >=70% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Luria Bertani HiVeg™ Agar, Miller (Miller Luria Bertani HiVeg™ Agar) |
|---|---|
| SKU | MV1151 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Lennox E.S., 1955, Transduction of Linked Genetic Characters of the host by bacteriophage P1., Virology, 1:190. |
| Customized Product Available | No |
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