Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for cultivation of members of the Enterobacteriaceae species and in culture of blood clots from patients with suspected enteric fever.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg® peptone | 20.000 |
| Synthetic detergent No. V | 5.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.0 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and add 1 ml of Streptokinase solution (100000 units/ml). Mix well and dispense into sterile tubes or flasks as desired.
Principle And Interpretation
Enterobacteriaceae inhabit a wide variety of niches that include the human gastrointestinal tract and various environmental niches. When blood samples from a patient with suspected enteric fever is submitted for the widal test, it is useful as a routine to culture the clot after separation of serum (1). If it is known that the blood has been withdrawn with strict aseptic precautions, the clot may be placed in a wide tube half-filled with broth, or in a wide mouth screw-capped bottle containing 80 ml of broth. When there is any doubt regarding the presence of contaminating organisms, and this is always a possibility when blood specimens are sent to the laboratory from a distance, the clot should be transferred directly to a tube of sterile ox bile and disintegrated with aseptic precautions. After overnight incubation the bile culture is examined for enteric organism in the usual manner. A method of clot culture with Streptokinase has been recommended (2). Blood is allowed to clot in 5 ml quantities in sterile screw-capped universal containers. The separated serum is removed and 15 ml of 0.5% Bile Broth Base with Streptokinase 100 units/ml is added to each bottle. The streptokinase causes rapid clot lysis with release of bacteria trapped in the clot (2)
Bile Broth Base, HiVeg® is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. It can be used for the cultivation and enumeration of bacteria which are not particularly fastidious.
HiVeg® peptone serves as a source of nitrogen, carbon, long chain amio acids and other essential amino acids. Synthetic detergent No. V inhibits majority of Gram-positive species. Sodium chloride maintains the isotonicity of the medium whereas addition of streptokinase solution causes rapid clot lysis with release of bacteria trapped in the clot (2).
Type of specimen
Clinical samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Yellow coloured, clear solution without any haziness
Reaction
Reaction of 3.0% w/v aqueous solution at 25°C. pH: 7.6±0.2
pH
7.40-7.80
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) |
Growth |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) |
50-100 | luxuriant |
| # Klebsiella aerogenes ATCC 13048 (00175*) |
50-100 | luxuriant |
| Salmonella Typhi ATCC 6539 |
50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) |
>=104 | inhibited |
Key: (*) Corresponding WDCM numbers (#) Formerly known as Enterobacter aerogenes
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Bile Broth Base, HiVeg™ |
|---|---|
| SKU | MV071 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Colle, J.G., Duguid J.P., Fraser A.G. and Marmion, B.P. (Eds.) 1989 Mackie and McCartney Practical Medical Microbiology,Vol. 2, p:134 Longman Group, UK. 2.Watson, K.C. 1955, Isolation of Salmonella Typhi from the blood stream. J. of ,,Lab and Clinical Medicine 46:128-134. |
| Customized Product Available | No |
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