Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Gms/Litre |
|---|---|
| Pancreatic digest of casein | 17.000 |
| Soybean peptone | 3.000 |
| Dextrose | 2.500 |
| Sodium chloride | 5.000 |
| Dipotassium phosphate | 2.500 |
| Final pH (at 25°C) | 7.3±0.1 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 25°C and store in cool dark place preferbly below 25°C.
Principle And Interpretation
Grove and Randall have elucidated the antibiotic assays and medias in their comprehensive treatise on antibiotic assays (1). Antibiotic assay Medium No. 37 is recommended for the cultivation of a wide variety of microorganisms and sterility testing of pharmaceutical preparations. This medium is also used for the sensitivity testing by the tube dilution method for antimicrobial agents (3). This medium is formulated in accordance with The United States Pharmacopoeia (2).
Turbidimetric antibiotic assay is based on the change or inhibition of growth of a test microorganisms in a liquid medium containing a uniform concentration of an antibiotic. After incubation of the test organism in the working dilutions of the antibiotics, the amount of growth is determined by measuring the light transmittance using spectrophotometer. The concentration of antibiotic is determined by comparing amounts of growth obtained with that given by the reference standard solutions. Use of this method is appropriate only when test samples are clear.
The combination of pancreatic digest of casein and Soybean peptone makes this medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose serves as the carbohydrate source and dipotassium phosphate faclitates buffering in the medium. Sodium chloride maintains the osmotic balance of the medium.
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate
Reaction: Reaction of 3.0% w/v aqueous solution. pH: 7.3±0.1
pH: 7.20-7.40
Cultural Response
MU1667: Cultural characteristics observed (i) for Bacteria at 30-35°C after 18-48 hours (ii) for Fungi at 20-25°C after 2-5 days
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant(when incubated anaerobically) |
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
| Candida albicans ATCC 10231 | 50-100 | luxuriant |
| Candida albicans ATCC 2091 | 50-100 | luxuriant |
| Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium No. 37 |
|---|---|
| SKU | MU1667 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Grove and Randall, 1955, Assay Methods of Antibiotics, Medical Encyclopedia, Inc. New York |
| Customized Product Available | No |
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