Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Antibiotic Assay Medium No. 36
Recommended for assay of Bleomycin & cultivation of a wide variety of microorganisms as well as for sterility testing in pharmaceutical procedures and in accordance with US Pharmacopoeia.
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Composition
| Ingredients | Gms/Litre |
|---|---|
| Pancreatic digest of casein | 15.000 |
| Papaic digest of soybean | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.1 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40 grams in 1000 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Grove and Randall have elucidated the antibiotic assays and medias in their comprehensive treatise on antibiotic assays (1). Antibiotic assay Medium No. 36 is recommended for preparation of inoculum of Mycobacterium smegmatis for the assay of Bleomycin. This medium is also used for the cultivation of a wide variety of microorganisms and sterility testing of pharmaceutical preparations. (3). This medium is formulated in accordance with The United States Pharmacopoeia (2).
The combination of pancreatic digest of casein and papaic digest of soybean makes this medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Sodium chloride maintains the osmotic balance of the medium
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light yellow coloured clear to slightly opalescent gel forms in Petri plates
pH: 7.20-7.40
Growth Promotion Test
In accordance with the harmonized method of USP.
Cultural Response
MU1666: Cultural characteristics observed after an incubation at 36-37.5°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Cultural Response | ||||
| Mycobacterium smegmatis ATCC 607 | 50-100 | good-luxuriant | >=50% | Bleomycin |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium No. 36 |
|---|---|
| SKU | MU1666 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Grove and Randall, 1955, Assay Methods of Antibiotics, Medical Encyclopedia, Inc. New York 2.United States Pharmacopoeia 1985, US Pharmacopoeial Convention, Inc.,Rockville, MD. |
| Customized Product Available | No |
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