Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Dey-Engley (D/E) Neutralizing Broth (without Bromo cresol purple)
Used in disinfectant testing where neutralizaton of antiseptics and disinfectants is important for determining its bactericidal activity in accordance with US Pharmacopoeia.
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Intended Use:
Used in disinfectant testing where neutralizaton of antiseptics and disinfectants is important for determining its bactericidal activity in accordance with USP.
Composition**
| Ingredients | g/L |
|---|---|
| Tryptone | 5.000 |
| Yeast extract | 2.500 |
| Dextrose (Glucose) | 10.000 |
| Sodium thioglycollate | 1.000 |
| Sodium thiosulphate | 6.000 |
| Sodium bisulphite | 2.500 |
| Lecithin | 7.000 |
| Polysorbate 80 (Tween 80) | 5.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 39.0 grams in 1000 ml purified/distilled water.Heat if necessary to dissolve the medium completely.Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Dey-Engley (D/E) Neutralizing Broth (without Bromo cresol purple) is formulated as per United States Pharmacopoeia (3). It neutralizes a broad spectrum of antiseptics and disinfectants including quaternary ammonium compounds, phenolics, iodine and chlorine preparations, mercurials, formaldehyde and glutaraldehyde. Sodium thioglycollate, sodium thiosulphate, sodium bisulphite, soya lecithin and polysorbate 80 act as neutralizing components.
Type of specimen
Pharmaceutical samples for sterility testing.
Specimen Collection and Handling:
For testing disinfectants, prepare two sets of test tubes, one containing 9 ml Dey-Engley Neutralizing Broth (MU1062) and other with 9 ml Dey-Engley Neutralizing Broth Base. Add 1 ml of disinfectant under test. Mix well and allow it to stand for 15 minutes. Inoculate 0.1 ml of 1:100,000 dilution of overnight broth cultures and incubate at 30-35°C for 48 hours Growth in Neutralizing Broth and no growth in Neutralizing Broth Base indicates neutralization of disinfectant. To check bactericidal activity, both broth tubes are inoculated on D/E Neutralizing Agar (M186). Positive growth from negative tubes of Neutralizing Broth Base indicates bacteriostatic substance while negative growth indicates a bactericidal disinfectant. All positive tubes should show growth on Dey-Engley Neutralizing Agar. The control disinfectants used in test procedure are 2% chlorine, 2%formaldehyde, 1% glutaraldehyde, 2% iodine, 2% phenol, 1/750 quaternary ammonium compounds, 1/1000 mercurials etc. After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- 1. Due to nutritional variations, some strains may show poor growth
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured opalescent solution
Growth Promotion Test
As per United States Pharmacopoeia
Cultural Response
Cultural characteristics observed after an incubation at i)For bacteria at 30-35°C for <=3 days i)For fungi at 20-25°C for <=5days.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| ** Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant |
Key:-*- Corresponding WDCM numbers
#Formerly known as Aspergillus niger
**Formerly known as Bacillus subtilis subsp. spizizenii
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | Dey-Engley (D/E) Neutralizing Broth (without Bromo cresol purple) |
|---|---|
| SKU | MU1062 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Engley and Dey, 1970. Chem. Spec. Manuf. Assoc. Proc., Mid-Year Meet., p. 100. |
| Customized Product Available | No |
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