Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for the detection of presence or absence of coliform bacteria from drinking water, recreational water, water from distribution or treatment plants, water from domestic tanks.
Directions
Collect 100 ml water to be tested in sterile disposable bottle. Add entire quantity of powder medium (PA Broth) slowly to water by swirling to dissolve the powder completely. After dissolution, incubate the bottles at 35 - 37 C for 24 - 48 hours. Observe the colour change of the medium from reddish- purple to yellow, indicating the presence of coliform bacteria.
Type of specimen
Water samples
Specimen Collection and Handling:
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(1) After use, contaminated materials must be sterilized by autoclaving before discarding.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control:
Appearance: Light yellow to greenish yellow coloured, homogenous, free flowing powder.
Colour and Clarity of solution: Reddish Purple coloured, clear solution, without any precipitate.
Cultural response: Cultural characteristics observed after an incubation at 35-37°C or 25-30°C for 24-48 hours..
| Organism (ATCC) | Growth | Colour of Medium |
|---|---|---|
| #Klebsiella aerogenes (13048) (00175*) | Good-luxuriant turbid | Light yellow |
| E.scherichia coli (25922) (00013*) | Good- luxuriant turbid | Yellow |
| Enterococcus faecalis (29212) (00087*) | inhibited | - |
| Klebsiella pneumoniae (13883) (00097*) | Good-luxuriant | Yellow |
| S. serotype Typhimurium (14028) )(00031*) | Good-luxuriant | Turbid purple |
| S. serotype Typhimurium (23564) | Good-luxuriant | Turbid purple |
Storage and Shelf Life
On receipt store between 10-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period
Disposal
Add few drops of disinfectant (Dettol, phenyl etc.) and discard the vial. Preferable to use the autoclave wherever the facility exists.
| Product Name | PA Coliform Kit |
|---|---|
| SKU | MS1186 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Weiss J.E. and Hunter C.A., 1939, J. Am. Water Works Assoc., 31 : 707. |
| Customized Product Available | No |
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