Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Pancreatic digest of gelatin | 7.000 |
| Yeast extract | 3.000 |
| Lactose monohydrate | 10.000 |
| Bile salts | 1.500 |
| Dextrose monohydrate | 10.000 |
| Sodium chloride | 5.000 |
| Neutral red | 0.030 |
| Crystal violet | 0.002 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 50.12 grams of dehydrated medium in 1000 ml purified /distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates..
Principle And Interpretation
It is selective medium recommended for detection of Enterobacteriaceae species as recommended by Indian Pharmacopoeia (1). Mossel et al (2,3,4) added dextrose to the medium observing improved detection of coliforms. Incubation can be carried out at different temperatures and incubation time depending upon the group of Enterobacteriaceae to be recovered (5).
Pancreatic digest of gelatin and yeast extract provide nitrogenous compounds and other nutrients essential for bacterial metabolism. This media is selective due to presence of the inhibitors; bile salts and crystal violet. Crystal violet inhibits gram-positive organisms especially Staphylococci. Neutral red indicator helps to detect lactose monohydrate and dextrose monohydrate fermentation. Lactose and glucose fermenting strains grow as red or pink and may be surrounded by a zone of acid precipitated bile. Sodium chloride maintains the osmotic equilibrium in the medium. The red colour is due to absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8.
Quality Control
Appearance: Light yellow to pink homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity of prepared medium: Reddish purple coloured clear to slightly opalescent gel forms in Petri plates.
pH: 7.10-7.50
Growth Promotion Test
Growth Promotion is carried out in accordance with Indian Pharmacopoeia and cultural characteristics are observed after an incubation at 35-37°C for 18-24 hours.
Cultural Response
| Organism | Inoculum (CFU) | Growth | Observed Lot Recovery value (CFU) | Recovery | Colour of colony |
|---|---|---|---|---|---|
| Escherichia coli ATCC 8739 | 50-100 | good-luxuriant | 25-100 | >=50% | pink-red |
| Escherichia coli NCTC 9002 | 50-100 | good-luxuriant | 25-100 | >=50% | pink-red |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | good-luxuriant | 25-100 | >=50% | light pink |
| Staphylococcus aureus ATCC 6538 | >=103 | inhibited | 0 | 0% | |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | 25-100 | >=50% | pink-red |
| Staphylococcus aureus ATCC 25923 | >=103 | inhibited | 0 | 0% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Medium 6, Crystal Violet, Neutral Red, Bile Agar with Dextrose |
|---|---|
| SKU | MM1684 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Indian Pharmacopoeia, 2007, Govt. of India, the controller of Publication, Delhi, India.2.Mossel D.A.A., Mengerink W.H.J. & Scholts H.H., 1962, J. Bacteriol, 84 : 381.3.Mossel D.A.A. et al, 1978, Lab. practice, 27 No. 12 : 10494.Mossel D.A.A. et al, 1979, Food Protect., 42 : 470.5.Mossel D.A.A. et al, 1986, J. Appl. Bact., 60 : 289 |
| Customized Product Available | No |
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