Blocking Buffer (10% BSA in 1X TBS)

Blocking buffer (10% BSA in 1XTBS) is made of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. The Blocking Buffer (10%) should be diluted up to 1% working concentration prior to use with 1X Tris-buffered saline. A good blocking buffer for a specific experiment will bind to all potential sites of nonspecific interaction, eliminating background without altering or obscuring the epitope for antibody binding. In case of immunoblotting/staining of phosphorylated protein TBS is recommended instead of PBS, because phosphate in PBS may interfere with immunoreaction between phospho protein and phospho specific Antibodies. This Blocking Buffer is a ready-to-use solution which can be used for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.