20X MOPS-SDS Running Buffer is formulated for running proteins on Bis-Tris gels only. This buffer is recommended for separating medium- to large-sized proteins.
20X MOPS-SDS Running Buffer is formulated for running proteins on Bis-Tris gels only. Bis-Tris gels are polyacrylamide gels designed to give optimal separation of small to medium sized proteins under denaturing conditions. It is composed of 1M MOPS, 1M Tris base, 2% SDS and 20mM EDTA. A reducing agent, Sodium bisulphite, has to be added in this buffer before electrophoresis. A reducing agent, Sodium bisulphite, has to be added in this buffer before electrophoresis.