Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Rapid HiEnterococci™ HiCynth™ Agar
Rapid HiEnterococci™ HiCynth™ Agar is recommended for identification and differentiation of Enterococci from water samples.
Your enquiry has been submitted
Please login to Post an Enquiry.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| HiCynth™ Peptone No.1 | 10.000 |
| Sodium chloride | 5.000 |
| Sodium azide | 0.300 |
| Chromogenic mixture | 0.060 |
| Polysorbate 80 | 2.000 |
| Disodium hydrogen phosphate | 1.250 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 33.61 gm in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45 - 50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Enterococci are commonly found in the faeces of humans and other warm-blooded animals. Although some strains are ubiquitous and not related to faecal pollution, the presence of Enterococci in water is an indication of faecal pollution and the possible presence of enteric pathogens. The Enterococci test is recommended as a measure of ambient fresh and marine recreational water quality. Epidemiological studies have lead to the development of criteria which can be used to promulgate recreational water standards based on established relationships between health effects and water quality. The significance of finding Enterococci in recreational fresh or marine water samples is the direct relationship between the density of Enterococci and the risk of gastrointestinal illness associated with swimming in water (1, 2). The Rapid HiEnterococci Agar allows for rapid identification and differentiation of Enterococci from water samples.
HiCynth™ Peptone No.1 supplies nitrogenous, carbonaceous compounds, long chain amino acids and other essential growth nutrients. Sodium chloride provides the osmotic balance for rapid growth of Enterococci. Sodium azide inhibits the accompanying microflora, especially the gram-negative organisms.The enzyme ẞ-D-glucosidase present in Enterococci cleaves the chromogenic substrate, resulting in a bblue green colour tofthe colonies.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured opalescent gel forms in Petri plates
Reaction
Reaction of 3.36% w/v aqueous solution at 25°C. pH: 7.5±0.2
pH
7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | none to poor | <=10% | |
| Enterococcus faecalis ATCC 29212 | 50-100 | good | 40-50% | blue green |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | none to poor | <=10% | |
| Staphylococcus aureus ATCC 25923 | 50-100 | good | 40-50% | colourless |
Storage and Shelf Life
Store below 30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on the label. Product performance is best if used within stated expiry period.
| Product Name | Rapid HiEnterococci™ HiCynth™ Agar |
|---|---|
| SKU | MCD1414 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1.Litsky W., Mallmann W. L., a Fifield C. W., 1953, Am. J. Pbl. Hlth.,43:873.2.Manafi M., Sommer R., 1993, Wat. Sci. Tech. 27:271-274. |
| Customized Product Available | No |
Write Your Own Review
HiCrome™ Enterococcus faecium HiVeg™ Agar Base
As low as
₹2,956.00
Rapid Hi-Enterococci Agar
As low as
₹2,714.00
HiCrome™ Enterococcus faecium Agar Base
As low as
₹2,755.00
HiCrome™ Enterococcus faecium HiCynth™ Agar Base
As low as
₹2,956.00
Upsell Products
Hiaureus™ Coagulase Confirmation Kit (w/o swabs)
As low as
₹5,331.00
Bile Esculin Agar Plate
As low as
₹1,074.00
Bile Esculin Azide Agar Plate
As low as
₹4,714.00
Baird Parker Agar Plate
As low as
₹2,095.00
Bile Esculin Azide Medium w/ Sterile Membrane Filter
As low as
₹10,950.00
Bile Esculin Azide Medium (Economy pack) (without Membrane Filter)
As low as
₹1,298.00
HiMotility Biochemical Kit for Vibrio parahaemolyticus
As low as
₹4,372.00
HiMotility Biochemical Kit for Vibrio cholerae
As low as
₹4,372.00
HiMotility™ Biochemical Kit for Listeria
As low as
₹4,543.00
HiMotility™ Biochemical Kit for Salmonella
As low as
₹4,543.00
HiMotility™ Biochemical Kit for E.coli
As low as
₹7,735.00
Bile Esculin Agar Slant in Tubes
As low as
₹1,192.00
Biochemical Test Kit for Salmonella
As low as
₹1,546.00
Biochemical Test Kit for Vibrio parahaemolyticus
As low as
₹5,416.00
Biochemical Test Kit for Vibrio cholerae
As low as
₹5,393.00
HiListeria™ Identification Kit
As low as
₹4,986.00
HiSalmonella™ Identification Kit
As low as
₹4,986.00
HiE. coli™ Identification Kit
As low as
₹4,986.00
HiVibrio™ Identification Kit
As low as
₹4,590.00
HiStaph™ Identification Kit
As low as
₹4,986.00
Bile Esculin Agar
As low as
₹1,192.00
HiCrome™ Salmonella Agar
As low as
₹9,145.00