Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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HiCrome™ Candida Differential HiCynth™ Agar
Recommended for rapid isolation and identification of Candida species from mixed cultures in clinical and non-clinical samples.
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Intended Use
HiCrome™ Candida Differential HiCynth™ Agar is recommended for rapid isolation and identification of Candida species from mixed cultures from various samples.
Composition
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ peptone No.2# | 15.000 |
| HiCynth™ peptone No.6# | 4.000 |
| Dipotassium hydrogen phosphate | 1.000 |
| Chromogenic mixture | 7.220 |
| Chloramphenicol | 0.500 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.3±0.2 |
Formula adjusted, standardized to suit performance parameters
# - Chemically defined peptones
Directions
Suspend 42.72 grams in 1000 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Perry and Miller (1) reported that Candida albicans produces an enzyme β-N-acetyl- galactosaminidase and according to Rousselle et al (2) incorporation of chromogenic or fluorogenic hexosaminidase substrates into the growth medium helps in identification of C.albicans isolates directly on primary isolation. HiCrome™ Candida Differential Agar is a selective and differential medium, which facilitates rapid isolation of yeasts from mixed cultures and allows differentiation of Candida species namely C.albicans, C.krusei, C.tropicalis and C.glabrata on the basis of colouration and colony morphology. On this medium results are obtained within 48 hours and it is useful for the rapid and presumptive identification of common yeasts in Mycology and Microbiology Laboratory. HiCrome™ Candida Differential HiCynth™ Agar is the modification of the same prepared by completely replacing animal and vegetable based peptones with chemically defined peptones to avoid BSE /TSE risks associated with animal peptones.
HiCynth™ peptone No.2 and HiCynth™ peptone No.6 provides nitrogenous, carbonaceous compounds and other essential growth nutrients. Phosphate buffers the medium well. Chloramphenicol suppresses the accompanying bacterial flora. C.albicans appear as light green coloured smooth colonies, C.tropicalis appear as blue to metallic blue coloured raised colonies. C.glabrata colonies appear as cream to white smooth colonies, while T.krusei appear as purple fuzzy colonies.
Type of specimen
Food samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Variations in colour intensity may be observed for Candida isolates depending on the presence of enzymes.
- Other Candida species may produce light mauve coloured colonies which is also produced by other yeast cells. This must be confirmed by further biochemical tests.
- Other filamentous fungi also exhibit colour on this medium.
Performance And Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to beige homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.27% w/v aqueous solution at 25°C. pH: 6.3±0.2
pH
6.10-6.50
Cultural Response
Cultural characteristics observed after an incubation at 30-35°C for 40-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | good-luxuriant | >=50% | light green |
| Candida glabrata ATCC 15126 | 50-100 | good-luxuriant | >=50% | cream to white |
| #Teunomyces krusei ATCC 24408 | 50-100 | good-luxuriant | >=50% | purple, fuzzy |
| Candida tropicalis ATCC 750 | 50-100 | good-luxuriant | >=50% | blue to purple |
| Candida kefyr ATCC 66058 | 50-100 | good-luxuriant | >=50% | cream to white with slight purple centre |
| Candida utilis ATCC 9950 | 50-100 | good-luxuriant | >=50% | pale pink to pinkish purple |
| Candida parapsilosis ATCC 22019 | 50-100 | good-luxuriant | >=50% | white to cream |
| Candida membranifaciens ATCC 20137 | 50-100 | good-luxuriant | >=50% | white to cream |
| Escherichia coli ATCC 25922 (00013*) | >=10⁴ | inhibited | 0% | |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=10⁴ | inhibited | 0% |
Key: (*) Corresponding WDCM numbers. (#) Formerly known as Candida krusei
Storage and Shelf life
Store between 15-25°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | HiCrome™ Candida Differential HiCynth™ Agar |
|---|---|
| SKU | MCD1297A |
| Product Type | HiCynth™, HiCrome™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | To be added |
| Customized Product Available | No |
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