Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Recommended for selective isolation of bile tolerant Lactobacilli.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 10.000 |
| Yeast extract | 5.000 |
| Potassium dihydrogen phosphate | 6.000 |
| Ammonium citrate | 2.000 |
| Dextrose (Glucose) | 20.000 |
| Polysorbate 80 (Tween 80) | 1.000 |
| Sodium acetate | 25.000 |
| Magnesium sulphate | 0.575 |
| Manganese sulphate | 0.120 |
| Ferrous sulphate | 0.034 |
| Bile # | 1.500 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Ox gall
Directions
Suspend 8.62 grams in 100 ml purified/distilled water. Add 0.132 ml glacial acetic acid. Heat to boiling to dissolve completely. Medium can be used without autoclaving. If storage is necessary, the medium can be autoclaved at 10 lbs pressure (115°C) for 15 minutes. Incubation is done in CO2 enriched atmosphere.
Principle And Interpretation
Rogosa SL Agar w/0.15% Bile is recommended for selective enumeration of bile tolerant fecal lactobacilli. Lactobacilli grow poorly on ordinary culture media and require special nutrients. It is a selective medium for isolation and enumeration of lactobacilli (1). The high acetate concentraion and low pH suppresses growth of many other strains of Lactic acid bacteria (2). Dextrose serves as energy source whereas Polysorbate 80 as source of fatty acids. Ammonium citrate and Sodium acetate inhibits moulds, Streptococci and many other organisms. Tryptone and Yeast extract provides the nitrogenous compounds. Magnesium sulphate, Manganese sulphate, Ferrous sulphate serves a trace elements for growth of Lactobacilli. Incorporation of 0.15% bile selectively allows the growth of bile tolerant Lactobacilli.
Type of specimen
Clinical samples - Saliva, faeces
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- High acetate concentration and acidic pH suppress many strains of other lactic acid bacteria.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow coloured homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light yellow coloured slightly opalescent gel forms in petri plates.
Reaction
Reaction of 8.62% w/v aqueous solution at 25°C. pH: 5.4±0.2
pH
5.20-5.60
Cultural Response
Cultural characteristics observed in presence of Carbon dioxide (CO2) after an incubation at 35 - 37°C after 48 hours
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | 0% |
| Lactobacillus acidophilus ATCC 4356 (00098*) | 50-100 | luxuriant | >=50% |
| ^Lactiplantibacillus plantarum ATCC 8014 | 50-100 | luxuriant | >=50% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | 0% |
Key: *Corresponding WDCM numbers. ^ Formerly known as Lactobacillus plantarum
Storage and Shelf Life
Store dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
Reference
- Rogosa M, Mitchell J.A. and Wiseman R.F, (1951), J. Bact. 62, 132 133.
- Mac Faddin J.F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol.I, Williams and Wilkins, Baltimore.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
| Product Name | Rogosa SL Agar w/ 0.15% Oxgall (Revised as Rogosa SL Agarw/ 0.15% Bile) |
|---|---|
| SKU | M958 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Rogosa M, Mitchell J.A. and Wiseman R.F, (1951), J. Bact. 62, 132 133. |
| Customized Product Available | No |
Write Your Own Review
ECD Agar
As low as
₹6,986.00
EC 0157:H7 Selective Broth (Twin Pack)
As low as
₹6,933.00
Tryptone Phosphate Broth
As low as
₹5,646.00