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C. botulinum Isolation Agar Base

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M911

For rapid, selective isolation of Clostridium botulinum from food samples.

Description

C. botulinum Isolation Agar Base is recommended for rapid, selective isolation of Clostridium botulinum from food samples. This medium is formulated as per the recommendation of APHA. Botulinal toxin is heat-labile. Therefore the test samples and cultures should be maintained at refrigeration temperatures. The pH of the toxic material should also be maintained at a slightly acidic pH since botulinal toxin is less stable at alkaline pH. Egg yolk emulsion added in the medium helps in detecting lecithinase, lipase and proteolytic activity. Lecithinase degrades lecithin present in the egg yolk producing an insoluble, opaque precipitate in the medium surrounding the growth . Lipase break down free fats present in the egg yolk causing an iridescent (oil on water) sheen to form on the surface of the colonies.

Product Information

More Information
Product Name	C. botulinum Isolation Agar Base
SKU	M911
Product Type	Regular
Physical Form	Powder
Origin	Animal
Packaging type	HDPE
References	1. Vanderzant C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination of foods,3rd Ed., APHA, Washington, D.C.2.Finegold S. M. and Baron E. J., 1986, Bailey and Scotts Diagnostic Microbiology, 7th Ed., The C.V. Mosby Company,St. Louis.3.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition.4.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.5.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C.6.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C.7.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.
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