Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for selective enrichment of Listeria species.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptose | 10.000 |
| Yeast extract | 5.000 |
| HM peptone B # | 5.000 |
| Sodium chloride | 20.000 |
| Disodium hydrogen phosphate | 9.600 |
| Potassium dihydrogen phosphate | 1.350 |
| Esculin | 1.000 |
| Nalidixic acid | 0.020 |
| Acriflavin hydrochloride (Trypaflavin) | 0.012 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 51.98 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.
Principle And Interpretation
Only Listeria monocytogenes among the Listeria species is reported to cause infection in humans. In human adults, L.monocytogenes primarily causes meningitis, encephalitis or septicemia. The tropism of L.monocytogenes for the central nervous system leads to severe disease, often with high mortality or with neurologic disorders among survivors (8). Listeria Enrichment Broth, Modified, a modification of the original formulation of Donnelly and Baigent, is used for the selective enrichment of Listeria species (2). In this medium, the nalidixic acid concentration has been reduced from 40 mg/l in the original composition, to 20 mg/l. Listeria Enrichment Broth, Modified is used for selective enrichment of Listeria species from milk, milk products and other foods.
This medium contains tryptose, yeast extract and HM peptone B which provide essential nutrients like carbon and nitrogenous compounds including vitamins, amino acids and trace ingredients. Phosphates provide buffering action to the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and acriflavin inhibit the growth of gram-negative and gram-positive organisms respectively (5,6,7) except Listeria species.
For enrichment, 25 gram or 25 ml sample is added to 225 ml medium in a stomacher bag. Homogenize the material if required. Incubation is carried out at 30°C for upto 7 days and the sample is subcultured on Listeria Selective Agar (M567) after 1, 2 and 7 days.
Type of specimen
Food and dairy samples.
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,9,10). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical identification of organisms is required for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Yellow coloured, clear to slightly opalescent solution having a bluish tinge
Reaction
Reaction of 5.2% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited |
| Listeria monocytogenes subsp. serovar 1 ATCC 19111 (00020*) | 50-100 | luxuriant |
| Listeria monocytogenes ATCC 19112 | 50-100 | luxuriant |
| Listeria monocytogenes ATCC 19117 | 50-100 | luxuriant |
| Listeria monocytogenes ATCC 19118 | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited |
Key: * - Corresponding WDCM numbers
Storage and Shelf Life
Store dehydrated and the prepared medium at 2-8° C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Listeria Enrichment Broth, Modified |
|---|---|
| SKU | M888 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. |
| Customized Product Available | No |
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