Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
6 MFA medium is used for cultivation of Aspergillus ochraceous.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Yeast extract | 10.000 |
| Potassium dihydrogen phosphate | 1.000 |
| Potassium chloride | 0.500 |
| Sodium nitrate | 3.000 |
| Magnesium sulphate | 0.500 |
| Ferrous sulphate | 0.010 |
| Zinc sulphate | 0.001 |
| Sodium potassium tartrate | 0.010 |
| Dextrose | 40.000 |
| Agar | 15.000 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 70.00 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour in sterile Petri plates.
Principle And Interpretation
Aspergillus is a large genus of common contaminants, containing about 175 species. Quite a diversity of species has been implicated in human mycoses (1), although these fungi normally inhabit other ecological niches. Aspergillus ochraceous was implicated in a case of antromycosis in humans (2)
Yeast extract provides a source of trace elements, vitamins and amino acids. Dextrose is the source of carbohydrate. Monopotassium dihydrogen phosphate and sodium potassium tartarate buffers the media. Magnesium sulphate, ferrous sulphate and zinc sulphate are sources of divalent cations and other necessary ions. Agar is the solidifying agent.
Quality Control
Appearance
Light yellow to yellow coloured homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates
Cultural Response
M866: Cultural characteristics observed after an incubation at 25-30°C for 24-48 hours.
| Organism | Growth |
|---|---|
| Aspergillus niger ATCC 16404 | good-luxuriant |
| Aspergillus ochraceous | good-luxuriant |
| Candida albicans ATCC 10231 | good-luxuriant |
| Saccharomyces cerevisiae ATCC 9763 | good-luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | 6 MFA Medium |
|---|---|
| SKU | M866 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal Free (Microbial) |
| Packaging type | HDPE |
| References | 1. Pitt,J.I.,199 |
| Customized Product Available | No |
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