Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Used for cultivation and isolation of Lactobacillus species.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 5.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 5.000 |
| Potassium dihydrogen phosphate | 0.500 |
| Dipotassium hydrogen phosphate | 0.500 |
| Magnesium sulphate | 0.250 |
| Manganese sulphate | 0.010 |
| Ferrous sulphate | 0.010 |
| Sodium chloride | 0.010 |
| Zinc sulphate | 0.001 |
| Copper sulphate | 0.001 |
| Cobalt sulphate | 0.001 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 31.28 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Lactobacilli grow in a variety of habitats, wherever high levels of soluble carbohydrate, protein background products, vitamins and a low oxygen tension occur (1). These sites include the oral cavity, the intestinal tract (2,3), the vagina (4), food products (5) and dairy products (6). PNY Medium is formulated for isolation and cultivation of Lactobacillus species. Peptone and yeast extract provide amino acids, other nitrogenous nutrients, vitamin B complex etc. Dextrose is the fermentable carbohydrate. The phosphates form buffering systemwhilesodium chloride maintains osmotic equilibrium. Other salts supply essential nutrients for the growth of the organisms.
Type of specimen
Clinical samples - Swabs from oral cavity, Faeces, etc.; Food and dairy samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (6,7). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow coloured homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 3.1% w/v aqueous solution at 25°C. pH: 6.0±0.2
pH
5.80-6.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours in presence of 3-5% CO2.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Lactobacillus casei ATCC 9595 | 50-100 | luxuriant |
| Lactobacillus leichmannii ATCC 4797 | 50-100 | luxuriant |
| Lactobacillus plantarum ATCC 8014 | 50-100 | luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | PNY Medium |
|---|---|
| SKU | M835 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Balows A., Truper H. G., Dworkin M., Harder W., Schleifer K. H., (Eds.), The Prokaryotes, 2nd Edi, 1992, Springer-Verlag2.Wiseman R. F, Sarles W. B, Benton D. A, Harper A. E and Elvehjem C.A., 1956, J. Bacteriol., 72:723.3.Ellis R. F. and Sarles W. B., 1958, J. Bacteriol., 75:272.4.Rogosa M. and Sharpe M. E., 1960, J. Gen. Microbiol., 23:1975.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,American Public Health Association, Washington, D.C.6.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C. |
| Customized Product Available | No |
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