Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Used for routine cultivation of non fastidious bacteria.
Composition**
| Ingredients | g/L |
|---|---|
| Peptone | 10.000 |
| HM peptone B# | 3.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 33.0 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour the medium in sterile Petri plates.
Principle And Interpretation
The majority of organisms to be studied in medical bacteriology are either pathogens or commensals of the human body, and in order to obtain suitable growth the artificial culture medium should provide nutrients and a pH (about 7.2) approximating to those of the tissues and body fluids. For routine purposes many of these nutrients are supplied by aqueous extracts of peptone, which is a product of the digestion of protein (1).
HM Peptone B Agar can be used as a general-purpose nutrient medium and is also recommended for preparation of pure culture of Candida species for carrying out fermentation studies (2). HM Peptone B Agar is a non-selective nutrient medium containing HM peptone B and peptone as a source of nitrogen and carbon and sodium chloride as a source of electrolytes.
Type of specimen
Clinical samples - urine, faeces, pus, etc.; Food and dairy sample; Water samples
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (5,6,7). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards. (8) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- This medium is general purpose medium and may not support the growth of fastidious organisms.
- Further biochemical and serological tests must be performed for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 3.3% w/v aqueous solution at 25°C. pH: 7.6±0.2
pH
7.40-7.80
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | >=70% |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store below 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | HM Peptone B Agar (Meat extract B Agar) |
|---|---|
| SKU | M806 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Collee J. G., Fraser A. G., Marimon B. P., Simmons A., (Eds.) ,1996, Mackie and McCartney Practical Medical Microbiology,14th Ed., Churchill Livingstone. |
| Customized Product Available | No |
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