Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for the presumptive identification of coliforms from large samples.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 40.000 |
| Lactose | 20.000 |
| Bile salts | 10.000 |
| Sodium chloride | 10.000 |
| Bromo cresol purple | 0.020 |
| Final pH (at 25°C) | 7.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 80.02 grams in 1000 ml purified/ distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes with inverted Durham tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
MacConkey Broth Purple (Double Strength) w/ BCP is a modification of MacConkey Medium (1). Childs and Allen (2) demonstrated the inhibitory effect of neutral red and therefore substituted it by the less inhibitory bromocresol purple dye. BCP is more sensitive in recording pH variation in the medium. Peptone provides essential growth nutrients. Lactose is the fermentable carbohydrate. Bile salts inhibit gram-positive organisms. Sodium chloride maintains the osmotic balance of the medium. Bromocresol purple is the pH indicator in the medium which turns yellow under acidic condition. Lactose fermention turn the medium yellow due to the acidity produced on lactose fermentation. The colour change of the dye is observed when the pH of the medium falls below 6.8. Lactose non-fermenting organisms like Salmonella and Shigella do not alter the appearance of the medium. Liquid specimens are directly inoculated while solids have to be homogenized in appropriate diluents such as physiological saline, phosphate buffers, etc. The inoculation must be effected at 10% v/v in Durhams tubes. If the inoculum is greater than 1 ml, it is necessary to use the medium at double strength, inoculating equal volumes of specimen and medium.
Type of specimen
Food and Dairy samples, Water samples.
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,4,5). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological testing must be carried out for further confirmation.
- The surface of the medium should be dry when inoculated.
Performance and Evaluation
Performace of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Light yellow to beige homogeneous free flowing powder
Colour and Clarity of prepared medium: Purple clear solution without any precipitate or scum
Reaction: Reaction of 8.0% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH: 7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Acid | Gas |
|---|---|---|---|---|
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | good-luxuriant | Positive reaction, yellow colour | Positive reaction |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant | Positive reaction, yellow colour | Positive reaction |
| Salmonella Choleraesuis ATCC 12011 | 50-100 | fair to good | Negative reaction, no colour change | Negative reaction |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | fair to good | Negative reaction, no colour change | Negative reaction |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited |
Key * -Corresponding WDCM numbers. # - Formerly known as Enterobacter aerogenes
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | MacConkey Broth Purple (Double Strength) w/ BCP |
|---|---|
| SKU | M796 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.MacConkey A. T., 1900, The Lancet, ii: 20.2.Childs E. and Allen, 1953, J. Hyg: Camb. 51:468-477 |
| Customized Product Available | No |
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