Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for cultivation of phytopathological and other fungi.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Lima beans, infusion (from 62.5 g) | 08.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 23 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Plant diseases are caused by a variety of living organisms (called pathogens) such as fungi, bacteria, viruses, nematodes, phytoplasmas, protozoa, and parasitic plants, and by non-living agents such as air pollutants, nutrient imbalances, and various environmental factors. Fungi are responsible for many diseases of plants, causing large losses in crop production. They are responsible for contaminating harvested crops with mycotoxins dangerous for public health. As such crops are the target of large-scale fungicide use, with the corresponding environmental and health hazards. Limabean Agar is recommended for cultivation of such phytopathological and other fungi (1).
Limabean Agar is composed of an infusion of dry lima beans and agar. Limabean infusion provides all essential growth nutrients for fungi. The pH of the medium is 5.6, which enables luxuriant fungal growth.
Type of specimen
Plant samples
Specimen Collection and Handling
For plant samples follow appropriate techniques for handling specimens as per established guidelines (2,3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured opalescent gel forms in Petri plates and may have slight precipitate.
Reaction
Reaction of 2.3% w/v aqueous solution at 25°C. pH: 5.6±0.2
pH
5.40-5.80
Cultural Response
Cultural characteristics observed after an incubation at 28-32°C for 40-48 hours.
| Organism | Growth |
|---|---|
| # Aspergillus brasiliensis ATCC 16404 (00053*) | luxuriant |
| Candida albicans ATCC 10231 (00054*) | luxuriant |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | luxuriant |
Key: (*) Corresponding WDCM numbers. (#) Formerly known as Aspergillus niger
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Limabean Agar |
|---|---|
| SKU | M736 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Atlas R. M., 2004, Handbook of Microbiological Media, Lawrence C. Parks (Ed.), 3rd Edition, CRC Press. |
| Customized Product Available | No |
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