EMB agar base is recommended asa basal medium to which different carbohydrates and other test substances may beadded for differentiation and study of various enteric bacteria. On fermentation of added carbohydrates, the organisms produces blue-black coloured colony with green metallic sheen. Eosin-Y and methylene blue are indicators in the medium. To a certain extent they also inhibit gram positive organisms.
1. Moffett M., Young D. and Stuart R. D., 1948, Brit, Med. J., 2:241. 2.Stuart S. D., Toshach S. R. and Patsula T. M., 1954, Can. J. Public Health, 45:73.