Welshimers Broth, Modified is a chemically defined minimal medium for the growth of Listeria monocytogenes.This medium is a modification of Welshimer's Broth, having additional amino acids L - Histidine and L- Tryptophan. All the aminoacids and vitamins act as the growth factors required by Listeria monocytogenes.
Ferric citrate stimulates the growth whereas the potassium and sodium phosphates provide buffering system. Glucose and glutamine are the primary sources of carbon and nitrogen.
1.Premaratne, R. J., W. Lin, and E. A. Johnson. 1991. Development of an improved chemical defined minimal medium forListeria monocytogenes. Appl. Environ. Microbiol. 57: 3046-3048.2.Welshimer,H.J. 1963.Vitamin requirements of Listeria monocytogenes J. Bacteriol. 85: 1156-1159.