Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Enrichment Broth for ECO157:H7 is used as an enrichment broth for the growth of E. coli O157:H7 from food and environmental samples.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Casein enzymic hydrolysate | 10.000 |
| Sorbitol | 10.000 |
| Bile salts mixture | 1.500 |
| Sodium chloride | 5.000 |
Final pH ( at 25°C): 7.1±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 26.5 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Mix well and dispense into sterile test tubes.
Principle And Interpretation
Enrichment Broth for ECO157:H7 is based on the formulation described by Rappaport and Henigh (1). Escherichia coli O157:H7 has been recognized as a cause of hemorrhagic colitis (2).
Casein enzymic hydrolysate provides nitrogenous, carbonaceous compounds and other essential growth nutrients. Sorbitol is a fermentable sugar, bile salt mixture inhibits most of the gram positive organisms.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow colouered clear solution without any precipitate
Reaction: Reaction of 2.65% w/v aqueous solution at 25°C. pH : 7.1±0.2
pH: 6.90-7.30
Cultural Response M1599: Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | luxuriant |
| Escherichia coli 0157:H7 NCTC 12900 | 50-100 | luxuriant |
| Enterococcus faecalis ATCC 29212 | >=103 | inhibited |
| Enterobacter sakazakii ATCC 12868 | 50-100 | luxuriant |
| Klebsiella pneumoniae ATCC 13883 | 50-100 | luxuriant |
| Staphylococcus aureus ATCC 25923 | >=103 | inhibited |
| Salmonella Enteritidis ATCC 13076 | 50-100 | luxuriant |
| Shigella flexneri ATCC 12022 | 50-100 | good |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Rappaport F and Henigh E., J. Clin. Path., 5:361.
- Karmali M. A., Petric M., Lim C., et al, 1985, J. Infect. Dis., 151:775.
| Product Name | Enrichment Broth for EC O157 : H7 |
|---|---|
| SKU | M1599 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Rappaport F and Henigh E., J. Clin. Path., 5:361.2.Karmali M. A., Petric M., Lim C., et al, 1985, J. Infect. Dis., 151:775. |
| Customized Product Available | No |
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