Kings Medium A is recommended for non-selective isolation, cultivation and pigment production of Pseudomonas species. It is particularly suited for the production of pyocyanin and pyorubin. Pigments and/ or their derivatives produced by Pseudomonas species play a role as siderophores in the iron uptake systems of bacteria, and hence, their production is markedly enhanced under conditions of iron deficiency. Pseudomonas aeruginosa produces blue-green pigment, pyocyanin.
1.King E. O., Ward M. K. and Raney D. E., 1954, J. Lab and Clin. Med., 44:301-307.2.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical Microbiology,ASM, Washington, D.C.