Minimum Salt w/ Acicase

Minimum Salt w/ Acicase is used for the cultivation of Escherichia coli strains used for genetic and molecular studies. The medium is prepared based on the formula originally suggested by Davis et al. Due to the addition of casein acid hydrolysate (acicase) it can be used for cultivating Escherichia coli strains used for genetic and molecular studies. Acicase supplies many amino acids (except tryptophan) to E. coli . Ammonium chloride is added as a nitrogen source. Dextrose serves as the carbon and energy source while the two phosphate salts buffers the medium against pH changes due to the utilization of carbohydrate. Magnesium ions are required in a variety of enzymatic reactions including DNA replication.