5X Minimum Salts

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M1253
Recommended for use in cultivation of recombinant strains of Escherichia coli.


5X Minimum Salts is recommended for use in cultivation of recombinant strains of Escherichia coli. This medium prepared based on the formulation of Davis et al and is used in cultivation of recombinant strains of Escherichia coli. Ammonium chloride is added as a nitrogen source. Glucose serves as the carbon and energy source while two phosphate salts buffers the medium against pH changes due to utilization of carbohydrate. Calcium and magnesium ions are required in a variety of enzymatic reactions including DNA replication. Sodium chloride maintains the osmotic balance.
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Product Name 5X Minimum Salts
SKU M1253
Product Type Regular
Physical Form Powder
Origin Chemically defined
Packaging type HDPE
References 1. Davis L. G., Dibner M. D., and Battery J. F., 1986, Basic Methods in Molecular Biology, Elsevier, New York.2.Sambrook J., Fritsch E. F. and Maniates T., 1989, Molecular Cloning : A Laboratory Manual, 2nd Ed., Cold Spring Harbour Laboratory, Cold Spring Harbour, N.Y.
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